For immunochemical detection of H4R3Me2s
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Histone H4R3Me2s Rabbit Monoclonal Antibody (Clone RM459)

Item No. 42794

Product Insert (PDF)
Technical Information
Synonyms
  • Symmetrically Dimethylated Histone H4 Arginine 3
Immunogen
A peptide corresponding to H4R3Me2s
Clone Designation
RM459
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
Multiplex-based assays, WB
Cross Reactivity
(+) H4R3Me2s(-) H4R3(-) H4R3Me1(-) H4R3Me2a(-) H3R2Me1(-) H3R2Me2a(-) H3R2Me2s(-) H3R8Me1(-) H3R8Me2a(-) H3R8Me2s
Species Reactivity
(+) Human(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

    Add
    Cayman Chemical
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    Product Description

    Histone H4 is one of four core histone proteins that are involved in the organization of DNA into chromatin.1 Histones are globular proteins with unstructured N-terminal tails and are subject to a variety of posttranslational modifications, such as methylation, acetylation, phosphorylation, and citrullination, that can influence chromatin structure and regulate gene transcription.1,2 Histone H4 symmetrical dimethylation at arginine 3 (H4R3Me2s) is regulated by protein arginine methyltransferase 5 (PRMT5) and PR domain zinc finger protein 4 (PRDM4).3 H4R3Me2s is enriched and associated with gene repression and cell growth in BGC-823 and SGC-7901 gastric cancer cells.4 Suppression of symmetrical dimethylation of H4R3 is associated with cardiomyocyte hypertrophy.5 Cayman's Histone H4R3Me2s Monoclonal Antibody (Clone RM459) can be used for multiplex-based assay and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Wang, Y., Li, M., Stadler, S., et al. Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation. J. Cell Biol. 184(2), 205-213 (2009).

    2. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    3. Cui, J.Y., Fu, Z.D., and Dempsey, J. The role of histone methylation and methyltransferases in gene regulation. Toxicoepigenetics Core Principles and Applications 31-84 (2018).

    4. Liu, M., Yao, B., Gui, T., et al. PRMT5-dependent transcriptional repression of c-Myc target genes promotes gastric cancer progression. Theranostics 10(10), 4437-4452 (2020).

    5. Cai, S., Wang, P., Xie, T., et al. Histone H4R3 symmetric di-methylation by Prmt5 protects against cardiac hypertrophy via regulation of Filip1L/β-catenin. Pharmacol. Res. 161, 105104 (2020).