Histone H3K36Me3 Rabbit Monoclonal Antibody (Clone RM491)

Histone 3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.¹ It is a globular protein that contains a histone fold domain with a C-terminal α-helix that facilitates nucleosome interactions and chromatin compaction, as well as an unstructured N-terminal tail that extends outside of the nucleosome core, both of which are subject to various post-translational modifications (PTMs), including ubiquitination, acetylation, methylation, and phosphorylation.^1,2,3 Histone H3 has a key role in transcriptional regulation.⁴ Increased nucleosome levels of trimethylated lysine 36 on histone 3 (H3K36Me3) are required for efficient DNA damage and nucleotide mismatch repair, DNA transcription, and repressing heterochromatin formation.^5,6,7 Increased tumor levels of histone H3K36Me3 are correlated with increased tumor size, frequency of lymph node metastasis, and survival in patients with esophageal squamous cell carcinoma.⁸ Cayman's Histone H3K36Me3 Rabbit Monoclonal Antibody (Clone RM491) can be used for ELISA, chromatin immunoprecipitation (ChiP), and multiplex-based assay applications.

WARNING This product is not for human or veterinary use.

1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

2. Wyrick, J.J., and Parra, M.A. The role of histone H2A and H2B post-translational modifications in transcription: A genomic perspective. Biochim. Biophys. Acta 1789(1), 37-44 (2009).

3. Wang, C.-Y., Hua, C.-Y., Hsu, H.-E., et al. The C-terminus of histone H2B is involved in chromatin compaction specifically at telomeres, independently of its monoubiquitylation at lysine 123. PLoS One 6(7), e22209 (2011).

4. Bhaumik, S.R., Smith, E., and Shilatifard, A. Covalent modifications of histones during development and disease pathogenesis. Nat. Struct. Mol. Biol. 14(11), 1008-1016 (2007).

5. Sun, Z., Zhang, Y., Jia, J., et al. H3K36me3, message from chromatin to DNA damage repair. Cell Biosci. 10, 9 (2020).

6. Lucio-Eterovic, A.K., Singh, M.M., Gardner, J.E., et al. Role for the nuclear receptor-binding SET domain protein 1 (NSD1) methyltransferase in coordinating lysine 36 methylation at histone 3 with RNA polymerase II function. Proc. Natl. Acad. Sci. USA 107(39), 16952-16957 (2010).

7. Harutyunyan, A.S., Chen, H., Lu, T., et al. H3K27M in gliomas causes a one-step decrease in H3K27 methylation and reduced spreading within the constraints of H3K36 methylation. Cell Rep. 33(7), 108390 (2020).

8. Zhou, M., Li, Y., Lin, S., et al. H3K9me3, H3K36me3, and H4K20me3 expression correlates with patient outcome in esophageal squamous cell carcinoma as epigenetic markers. Dig. Dis. Sci. 64(8), 2147-2157 (2019).