Testosterone Rabbit Monoclonal Antibody (Clone RM435)

Testosterone is the prototypic and predominant circulating androgenic steroid.^1,2 It plays a major role in the growth and function of many reproductive and non-reproductive tissues and organs including muscle, liver, and brain, directing the development of the male phenotype during embryogenesis at puberty.¹ Testosterone is synthesized from pregnenolone by two main pathways, the Δ⁴ and Δ⁵ pathways. Both of these pathways involve the same three enzymes: the cytochrome P450 (CYP) isoform CYP17, 3β-hydroxysteroid dehydrogenase (3β-HSD), and 17β-HSD. The human CYP17 favors the Δ⁵ pathway, in which 17-hydroxypregnenolone is converted first to dehydroepiandrosterone (DHEA), then androstenedione, and finally testosterone. The Δ⁴ pathway in which 17-hydroxyprogesterone is converted to androstenedione is also important, but less favored in humans. While testosterone is the androgen responsible for biological activity in tissues such as muscle, in other tissues including gonads and skin, it is converted to 5α-DHT by the action of 5α-reductase. Cayman's Testosterone Rabbit Monoclonal Antibody (Clone RM435) can be used for ELISA.

WARNING This product is not for human or veterinary use.

1. Kicman, A.T. Biochemical and physiological aspects of endogenous androgens. Handb. Exp. Pharmacol. 195, 25-64 (2010).

2. Gower, D.B. Analysis of androgens and their derivatives. Steroid Analysis 457-558 (2010).