For immunochemical detection of DDDDK-tagged proteins
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DDDDK-tag Rabbit Monoclonal Antibody (Clone RM399)

Item No. 42811

Product Insert (PDF)
Technical Information
Immunogen
A mixture of peptides N- or C-terminally labeled with a DDDDK-tag
Clone Designation
RM399
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
WB
Cross Reactivity
(+) Recombinant proteins containing a DDDDK-tag(-) Endogenous mammalian proteins(-) Endogenous bacterial proteins
Species Reactivity
(Independent) Species
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    100 µg

    $438

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    Product Description

    DDDDK-tag Rabbit Monoclonal Antibody is a probe for the immunochemical detection of DDDDK tags on recombinant proteins. Recombinant proteins are commonly labeled with affinity tags, such as DDDDK, which is a derivative of the DYKDDDDK-tag and a recognition sequence for enterokinases, to facilitate both their detection and purification.1,2,3. DDDDK tags are utilized because of their small size, low potential to interfere in protein folding or activity, and strong immunoreactivity. Cayman's DDDDK-tag Rabbit Monoclonal Antibody (Clone RM399) can be used for Western blots (WBs).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hopp, T.P., Prickett, K.S., Price, V.L., et al. A short polypeptide marker sequence useful for recombinant protein identification and purification. Nat. Biotechnol. 6, 1204-1210 (1988).

    2. Terpe, K. Overview of tag protein fusions: From molecular and biochemical fundamentals to commercial systems. Appl. Microbiol. Biotechnol. 60(5), 523-533 (2003).

    3. Ferrando, R.E., Newton, K., Chu, F., et al. Immunohistochemical detection of FLAG-tagged endogenous proteins in knock-in mice. J. Histochem. Cytochem. 63(4), 244-255 (2015).