Histone H3^K4M Rabbit Monoclonal Antibody (Clone RM363)

Histone 3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.¹ It is a globular protein that contains a histone fold domain with a C-terminal α-helix that facilitates nucleosome interactions and chromatin compaction, as well as an unstructured N-terminal tail that extends outside of the nucleosome core, both of which are subject to various post-translational modifications (PTMs), including ubiquitination, acetylation, methylation, and phosphorylation.^1,2,3 Histone H3 has a key role in transcriptional regulation.⁴ Expression of histone H3.3, a subtype of histone 3, containing a lysine-to-methionine substitution at residue 4 (H3.3^K4M) decreases the levels of histone H3K4Me1 and H3K4Me3 in primary mouse cortical neurons.⁵ Cayman's Histone H3 K4M Mutant Rabbit Monoclonal Antibody (Clone RM363) can be used for ELISA, immunohistochemistry (IHC), and Western blot (WB) applications.

WARNING This product is not for human or veterinary use.

1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

2. Wyrick, J.J., and Parra, M.A. The role of histone H2A and H2B post-translational modifications in transcription: A genomic perspective. Biochim. Biophys. Acta 1789(1), 37-44 (2009).

3. Wang, C.-Y., Hua, C.-Y., Hsu, H.-E., et al. The C-terminus of histone H2B is involved in chromatin compaction specifically at telomeres, independently of its monoubiquitylation at lysine 123. PLoS One 6(7), e22209 (2011).

4. Bhaumik, S.R., Smith, E., and Shilatifard, A. Covalent modifications of histones during development and disease pathogenesis. Nat. Struct. Mol. Biol. 14(11), 1008-1016 (2007).

5. Warren, S., Xiong, S., Robles-Magallanes, D., et al. A vector system encoding histone H3 mutants facilitates manipulations of the neuronal epigenome. Sci. Rep. 14(1), 24415 (2024).