For immunochemical detection of MLC2 (phospho-Ser19)
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

MLC2 (Phospho-Ser19) Rabbit Monoclonal Antibody (Clone RM484)

Item No. 42816

Product Insert (PDF)
Technical Information
Synonyms
  • MLC-2v
  • MYL2
  • Myosin Light Chain 2
  • Myosin Regulatory Light Chain 2
  • RLC2
  • Ventricular Myosin Light Chain 2
Immunogen
A phospho-peptide corresponding to human Phospho-Myl2 (Ser19)
Clone Designation
RM484
MW
18.29 kDa
Formulation (Request formulation change)
100 µl of protein A affinity-purified recombinant monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
WB
Cross Reactivity
(+) MCL2 (phospho-Ser19)(-) Unphosphorylated MCL2
Species Reactivity
(+) Human(+) Mouse(+) Rat
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

    Add
    Product Description

    Myosin light-chain 2 (MLC2), also known as myosin regulatory light chain 2, is a subtype of MLC expressed in cardiac ventricular and skeletal slow-twitch muscles.1,2 MLC2 is composed of three calcium-binding EF-hand domains and is found in the head-stem interface of myosin with other MLC isoforms.2 It is phosphorylated at serine 19 (Ser19) by MLC kinase (MLCK), which stabilizes myosin filaments for cardiac- or skeletal muscle contractions.3,4 Mutations in MYL2, the gene encoding MLC2, have been found in patients with mild familial hypertrophic cardiomyopathy (FHC).5 Cayman's MCL2 (Phospho-Ser19) Rabbit Monoclonal Antibody (Clone RM484) can be used for Western blot (WB).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Sweeney, H.L., Bowman, B.F., and Stull, J.T. Myosin light chain phosphorylation in vertebrate striated muscle: Regulation and function. Am. J. Physiol. 264(5 Pt 1), C1085-1095 (1993).

    2. Szczesna, D. Regulatory light chains of striated muscle myosin. Structure, function and malfunction. Curr. Drug Targets Cardiovasc. Haematol. Disord. 3(2), 187-197 (2003).

    3. Adhikari, B.B., and Wang, K. Interplay of troponin- and myosin-based pathways of calcium activation in skeletal and cardiac muscle: The use of W7 as an inhibitor of thin filament activation. Biophys. J. 86(1), 359-370 (2004).

    4. Regueiro, J., Olguín, N., Simal-Gándara, J., et al. Toxicity evaluation of new agricultural fungicides in primary cultured cortical neurons. Environ. Res. 140, 37-44 (2015).

    5. Andersen, P.S., Havndrup, O., Bundgaard, H., et al. Myosin light chain mutations in familial hypertrophic cardiomyopathy: Phenotypic presentation and frequency in Danish and South African populations. J. Med. Genet. 38(12), E43 (2001).