For immunochemical detection of SMAD1 (phospho-Ser463,465)/SMAD5 (phospho-Ser463,465)/SMAD9 (phospho-Ser465,467)
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SMAD1 (Phospho-Ser463,465)/SMAD5 (Phospho-Ser463,465)/SMAD9 (Phospho-Ser465,467) Rabbit Monoclonal Antibody (Clone RM487)

Item No. 42817

Product Insert (PDF)
Technical Information
Immunogen
Phosphopeptide corresponding to of SMAD1 (phospho-Ser463,465)/SMAD5 (phospho-Ser463,465)/SMAD9 (phospho-Ser465,467)
Clone Designation
RM487
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
WB
Cross Reactivity
(+) SMAD1 (phospho-Ser463,465)/SMAD5 (phospho-Ser463,465)/SMAD9 (phospho-Ser465,467)(-) SMAD1/SMAD5/SMAD9 without phosphorylation
Species Reactivity
(+) Human(+) Mouse(+) Rat
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

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    Product Description

    SMAD proteins are intracellular transcription factors and mediators of TGF-β signaling.1,2 They are composed of an N-terminal MAD homology 1 (MH1) domain containing nuclear localization and DNA-binding sequences and a C-terminal MH2 domain that is essential for SMAD-receptor interactions, as well as SMAD-SMAD interactions. Binding of TGF-β or bone morphogenetic protein (BMP) to their respective cell surface receptors initiates recruitment and activation of SMAD proteins. TGF-β induces SMAD2 and SMAD3 activation, while BMP binding induces activation of SMAD1, SMAD5, and SMAD9 via phosphorylation of serine 463 and 465 (Ser463,465) in SMAD1 and SMAD5 and Ser465,467 in SMAD9.1,2,3 SMAD1, -5, and -9 activation is associated with hypertrophy in patients with osteoarthritis, but associated with inhibition of fibrotic gene expression in various fibrotic diseases, including chronic kidney disease (CKD).3,2 Cayman’s SMAD1 (Phospho-Ser463,465)/SMAD5 (Phospho-Ser463,465)/SMAD9 (Phospho-Ser465,467) Rabbit Monoclonal Antibody (Clone RM487) can be used for Western blot (WB).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lai, L.Y.S., Gracie, N.P., Gowripalan, A., et al. SMAD proteins: Mediators of diverse outcomes during infection. Eur. J. Cell Biol. 101(2), 151204 (2022).

    2. Hu, H.H., Chen, D.Q., Wang, Y.N., et al. New insights into TGF-β/Smad signaling in tissue fibrosis. Chem. Biol. Interact. 292, 76-83 (2018).

    3. Thielen, N.G.M., van Caam, A.P.M., v. Beuningen, H.M., et al. Separating friend from foe: Inhibition of TGF-β-induced detrimental SMAD1/5/9 phosphorylation while maintaining protective SMAD2/3 signaling in OA chondrocytes. Osteoarthritis Cartilage 31(11), 1481-1490 (2023).