For immunochemical detection of cleaved caspase-8
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Caspase-8 (Cleaved) Rabbit Monoclonal Antibody (Clone RM442)

Item No. 42833

Product Insert (PDF)
Technical Information
Synonyms
  • CAP4
  • CASP-8
  • Apoptosis Protease Mch-5
  • FADD-homologous ICE/ced-3-like Protease
  • FADD-like ICE
  • FLICE
  • ICE-like Apoptotic Protease 5
  • MACH
  • MORT-1-associated ced-3 Homolog
Immunogen
A peptide corresponding to the internal region of caspase 8
Clone Designation
RM-442
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
WB
Cross Reactivity
(+) Human cleaved caspase-8
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $411

    Add
    Cayman Chemical
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    Product Description

    Caspase-8 is an initiator caspase with roles in cell death, including induction of apoptosis and pyroptosis and inhibition of necroptosis.1 It is widely expressed during development and adulthood with high levels of expression in the heart and limb buds, as well as other tissues, in development and in leukocytes in adulthood.2 It localizes to the cytosol and can be found in the lamella of migrating cells.3 Caspase-8 is produced as an inactive proenzyme containing a prodomain, which is composed of two N-terminal death-effector domains (DEDs), and two C-terminal catalytic subunits: p18 and p10.4,1 Activation of the proenzyme occurs when it binds to the death-inducing signaling complex (DISC), which is composed of CD95, a Fas-associated death domain (FADD) protein, and cellular FLICE-like inhibitory protein (c-FLIP). Upon DISC binding, procaspase-8 is cleaved to release the p10 subunit, then the p18 subunit. Together, two of each subunit form an activated and stabilized heterotetramer, which cleaves other procaspases, including procaspase-3 and procaspase-7, to initiate the extrinsic apoptosis pathway. Caspase-8 also has a role in the innate immune response via its activation by toll-like receptor 4 (TLR4) signaling and its initiation of the pyroptosis pathway through the NOD-like receptor protein 3 (NLRP3) inflammasome.1 Mutations in CASP8, the gene encoding caspase-8, or dysfunctional caspase-8 activity are associated with a variety of conditions, including autoimmune disorders, immunodeficiencies, and an increased risk of cancer. Cayman’s Caspase-8 (Cleaved) Rabbit Monoclonal Antibody (Clone RM442) be used for Western blot (WB).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Pang, J., and Vince, J.E. The role of caspase-8 in inflammatory signalling and pyroptotic cell death. Semin. Immunol. 70, 101832 (2023).

    2. Kruidering, M., and Evan, G.I. Caspase-8 in apoptosis: The beginning of "the end"? IUBMB Life 50(2), 85-90 (2000).

    3. Keller, N., Ozmadenci, D., Ichim, G., et al. Caspase-8 function, and phosphorylation, in cell migration. Semin. Cell Dev. Biol. 82, 105-117 (2018).

    4. Ivanisenko, N.V., and Lavrik, I.N. Mechanisms of procaspase-8 activation in the extrinsic programmed cell death pathway. Mol. Biol. (Mosk) 53(5), 830-837 (2019).