For immunochemical detection of DDIT3
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DDIT3 Rabbit Monoclonal Antibody (Clone RM485)

Item No. 42841

Product Insert (PDF)
Technical Information
Synonyms
  • CHOP
  • C/EBPζ
  • C/EBP-homologous Protein
  • DNA Damage-inducible Transcript 3
  • DNA Damage-Induced Gene 153
  • Growth Arrest and DNA Damage-Inducible Protein 153
  • GADD153
Immunogen
Peptide corresponding to the N-terminus of human DDIT3
Clone Designation
RM485
Formulation (Request formulation change)
100 µl of protein A-affinity purified antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Rabbit
Isotype
IgG
Applications
WB
Cross Reactivity
(+) DDIT3
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $411

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    Product Description

    DNA damage-inducible transcript 3 (DDIT3), also known as C/EBP-homologous protein (CHOP), is a transcription factor and member of the CCAAT/enhancer-binding protein (C/EBP) family.1,2 It is composed of an N-terminal transactivation domain and a C-terminal leucine zipper domain containing a DNA-binding region.2 DDIT3 is ubiquitously expressed and found in the cytosol at low levels.1 It is phosphorylated at serine 78 (Ser78) and Ser81 in response to endoplasmic reticulum (ER) stress, such as low calcium, glucose, or amino acid levels, and enters the nucleus and forms heterodimers with C/EBP family members to either promote or inhibit gene expression.1,3 DDIT3 induces apoptosis in response to calcium store depletion induced by nitric oxide (NO) in primary mouse pancreatic β-cells.4 It induces macrophage M1 polarization and increases tooth pulp inflammation in a mouse model of pulpitis.5 Knockdown of DDIT3 restores erythropoiesis in hematopoietic stem cells isolated from patients with anemia.6 Cayman's DDIT3 Rabbit Monoclonal Antibody (Clone RM485) can be used for Western blot (WB).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Oyadomari, S., and Mori, M. Roles of CHOP/GADD153 in endoplasmic reticulum stress. Cell Death Differ. 11(4), 381-389 (2004).

    2. Poli, V. The role of C/EBP isoforms in the control of inflammatory and native immunity functions. The Journal of Biological Chemisty 273(45), 29279-29282 (1998).

    3. Marwarha, G., Rostad, S., Lilek, J., et al. Palmitate increases β-site AβPP-cleavage enzyme 1 activity and amyloid-β genesis by evoking endoplasmic reticulum stress and subsequent C/EBP homologous protein activation. J. Alzheimers Dis. 57(3), 907-925 (2017).

    4. Oyadomari, S., Takeda, K., Takiguchi, M., et al. Nitric oxide-induced apoptosis in pancreatic beta cells is mediated by the endoplasmic reticulum stress pathway. Proc. Natl. Acad. Sci. USA 98(19), 10845-10850 (2001).

    5. Wang, Y., He, Y., Dong, W., et al. DDIT3 aggravates pulpitis by modulating M1 polarization through EGR1 in macrophages. Int. Immunopharmacol. 120, 110328 (2023).

    6. Berastegui, N., Ainciburu, M., Romero, J.P., et al. The transcription factor DDIT3 is a potential driver of dyserythropoiesis in myelodysplastic syndromes. Nat. Commun. 13(1), 7619 (2022).