CAIX Rabbit Monoclonal Antibody (Clone RM486)

Carbonic anhydrase IX (CAIX) is a transmembrane carbonic anhydrase and a member of the carbonic anhydrase family.^1,2 It is composed of an N-terminal proteoglycan-like domain, a catalytic domain, a transmembrane region, and a C-terminal cytosolic tail.³ CAIX is expressed in the epithelia of the gastrointestinal tract where it catalyzes the reversible zinc-dependent hydrolysis of carbon dioxide (CO₂) and maintains intracellular pH homeostasis.^1,2 The levels of CAIX are increased in chronically hypoxic tumors, which contributes to the acidification of the extracellular matrix (ECM) by decreasing elevated intracellular CO₂ levels produced from increased metabolism.² CAIX inhibition decreases metastasis and increases hypoxic stress in mouse breast cancer xenograft models.⁴ High tumor levels of CAIX are correlated with distant metastasis and poor survival in patients with breast cancer.⁵ Cayman's CAIX Rabbit Monoclonal Antibody (Clone RM486) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

WARNING This product is not for human or veterinary use.

1. Supuran, C.T., and Scozzafava, A. Carbonic anhydrases as targets for medicinal chemistry. Bioorg. Med. Chem. 15(13), 4336-4350 (2007).

2. Becker, H.M. Carbonic anhydrase IX and acid transport in cancer. Br. J. Cancer. 122(2), 157-167 (2020).

3. Vullo, D., Franchi, M., Gallori, E., et al. Carbonic anhydrase inhibitors: Inhibition of the tumor-associated isozyme IX with aromatic and heterocyclic sulfonamides. Bioorg. Med. Chem. Lett. 13(6), 1005-1009 (2003).

4. Gieling, R.G., Babur, M., Mamnani, L., et al. Antimetastatic effect of sulfamate carbonic anhydrase IX inhibitors in breast carcinoma xenografts. J. Med. Chem. 55(11), 5591-5600 (2012).

5. Lou, Y., McDonald, P.C., Oloumi, A., et al. Targeting tumor hypoxia: suppression of breast tumor growth and metastasis by novel carbonic anhydrase IX inhibitors. Cancer Res. 71(9), 3364-3376 (2011).