For immunochemical detection of TIA-1
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TIA-1 Rabbit Monoclonal Antibody (Clone RM490)

Item No. 42844

Product Insert (PDF)
Technical Information
Synonyms
  • Cytotoxic Granule-associated RNA-Binding Protein TIA-1
  • Nucleolysin TIA-1 Isoform p40
  • T-cell-restricted Intracellular Antigen-1
Immunogen
A peptide corresponding to the C-terminus of human TIA-1
Clone Designation
RM490
Formulation (Request formulation change)
100 µl of protein A affinity-purified recombinant monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
WB
Cross Reactivity
(+) TIA-1
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    100 µl

    $411

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    Product Description

    T-cell-restricted intracellular antigen-1 (TIA-1) is an RNA-binding protein that plays a role in pre-mRNA splicing, RNA metabolism, and stress granule formation.1 It is composed of three RNA recognition motifs (RRMs) and a C-terminal glutamine-rich auxiliary region, where RRM3 mediates nuclear export and RRM2 and part of the C-terminal region mediate nuclear import.2,3 TIA-1 exists as two isoforms, is ubiquitously expressed, and localizes to the cytoplasm and nucleus. It is involved in the regulation of diverse biological processes, including transcription, translation, stress response, apoptosis, and autophagy, among others.1 Mutations in TIA-1 are associated with Welander distal myopathy, amyotrophic lateral sclerosis (ALS), and ALS with frontotemporal dementia (FTD).4,5 Cayman’s TIA-1 Rabbit Monoclonal Antibody (Clone RM490) can be used for Western blot (WB).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Fernández-Gómez, A., and Izquierdo, J.M. The multifunctional faces of T-cell intracellular antigen 1 in health and disease. Int. J. Mol. Sci. 23(3), 1400 (2022).

    2. Izquierdo, J.M., and Valcárcel, J. Two isoforms of the T-cell intracellular antigen 1 (TIA-1) splicing factor display distinct splicing regulation activities. Control of TIA-1 isoform ratio by TIA-1-related protein. Comparative Study 282(27), 19410-19417 (2007).

    3. Zhang, T., Delestienne, N., Huez, G., et al. Identification of the sequence determinants mediating the nucleo-cytoplasmic shuttling of TIAR and TIA-1 RNA-binding proteins. J. Cell Sci. 118(Pt 23), 5453-5463 (2005).

    4. Hackman, P., Sarparanta, J., Lehtinen, S., et al. Welander distal myopathy is caused by a mutation in the RNA-binding protein TIA1. Ann. Neurol. 73(4), 500-509 (2013).

    5. Mackenzie, I.R., Nicholson, A.M., Sarkar, M., et al. TIA1 mutations in amyotrophic lateral sclerosis and frontotemporal dementia promote phase separation and alter stress granule dynamics. Neuron. 94(4), 808-816 (2017).