CD68 Rabbit Monoclonal Antibody (Clone RM496)

CD68, also known as macrosialin, is a lysosome-associated membrane protein (LAMP).¹ It is composed of an N-terminal cleavable signal peptide, a mucin-like domain, which is heavily glycosylated, a proline hinge region, a LAMP-like domain, a transmembrane domain, and a C-terminal cytoplasmic tail.² CD68 localizes to endosomal and lysosomal membranes but can also be localized in the plasma membrane.¹ It is expressed primarily in mononuclear phagocytes, such as macrophages, microglia, osteoclasts, and myeloid dendritic cells, but can also be expressed to a lesser extent in CD19⁺ B cells and CD4⁺ T cells.² CD68 overexpression in tumor cells is associated with immune evasion through the prevention of immune cell contact. It has been used as a non-specific cell surface marker for macrophages and tumor-associated macrophages (TAMs).^1,3,2 Cd68⁺ macrophages levels are increased in mouse brain in a model of Gaucher's disease, a condition characterized by a lack of the glycolipid-metabolizing enzyme glucosylceramidase.⁴ High tumor levels of CD68 are correlated with poor prognosis in patients with glioblastoma multiforme (GBM) and thymoma.⁵ Cayman's CD68 Rabbit Monoclonal Antibody (Clone RM496) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

WARNING This product is not for human or veterinary use.

1. Alessandrini, F., Pezzè, L., and Ciribilli, Y. LAMPs: Shedding light on cancer biology. Semin. Oncol. 44(4), 239-253 (2017).

2. Chistiakov, D.A., Killingsworth, M.C., Myasoedova, V.A., et al. CD68/macrosialin: Not just a histochemical marker. Lab. Invest. 97(1), 4-13 (2017).

3. Zhao, X., Gao, M., He, J., et al. Perilipin 1 deficiency in whole body or bone marrow-derived cells attenuates lesions in atherosclerosis-prone mice. PLoS One 10(4), e0123738 (2015).

4. Marshall, J., Sun, Y., Bangari, D.S., et al. CNS-accessible inhibitor of glucosylceramide synthase for substrate reduction therapy of neuronopathic Gaucher disease. Mol. Ther. 24(6), 1019-1029 (2016).

5. Zhang, J., Li, S., Liu, F., et al. Role of CD68 in tumor immunity and prognosis prediction in pan-cancer. Sci. Rep. 12(1), 7844 (2022).