CD45 Rabbit Monoclonal Antibody (Clone RM497)

CD45 is a type I transmembrane glycoprotein that is encoded by the PTPRC gene in humans and is abundantly expressed on nucleated hematopoietic cells.¹ It contains an extracellular region that binds to glycoproteins and exists in various isoforms in a cell type-, differentiation state-, and activation state-dependent manner.^1,2 CD45 also contains two highly conserved cytoplasmic protein tyrosine phosphatase domains that regulate intracellular signaling.¹ CD45 functions as a negative or positive regulator of antigen receptor signaling in T and B cells through dephosphorylation of Src family kinases in a cell type- and differentiation state-dependent manner.² It also dephosphorylates JAK kinases, inhibiting cytokine and chemokine signaling in leukocytes. Mutations in PTPRC are associated with severe combined immunodeficiency (SCID) in mice and humans.^3,4,5 The number of CD45⁺ cells is increased in postmortem brain from patients with Alzheimer's disease.⁶ Increased levels of the CD45 isoform CD45RO have been found on CD19⁺ lamina propria B cells isolated from patients with Crohn's disease.² Fluorescently labeled versions of CD45 have commonly been used in flow cytometry as a pan leukocyte marker and to identify certain subsets of hematopoietic stem cells (HSCs).⁷ Cayman's CD45 Rabbit Monoclonal Antibody (Clone RM497) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

WARNING This product is not for human or veterinary use.

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