For immunochemical detection of VISTA
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VISTA Rabbit Monoclonal Antibody (Clone RM503)

Item No. 42854

Product Insert (PDF)
Technical Information
Synonyms
  • B7-H5
  • PD-1H
  • Platelet Receptor Gi24
  • Programmed Death-1 Homolog
  • SISP1
  • Stress-induced Secreted Protein-1
  • V-domain Ig Suppressor of T cell Activation
  • V-set Immunoregulatory Receptor
  • VSIR
  • V-type Immunoglobulin Domain-containing Suppressor of T cell Activation
Immunogen
A peptide corresponding to residues near the C-terminus of human VISTA
Clone Designation
RM503
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) VISTA
Species Reactivity
(+) Human(+) Mouse(+) Rat
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

    Add
    Cayman Chemical
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    Product Description

    V-type immunoglobulin domain-containing suppressor of T cell activation (VISTA) is a type I transmembrane protein and negative immune checkpoint regulator.1,2,3,4 It is composed of an N-terminal immunoglobulin (Ig) V domain, a stalk domain, a transmembrane domain, and a cytoplasmic domain.2 VISTA is primarily expressed in myeloid lineage hematopoietic cells, as well as the spleen, placenta, and other tissues with infiltrating leukocytes.3,4,5 It is involved in immunosuppression of T cell activation and acts as a ligand and receptor.1,2,3,4 Increased protein levels of VISTA in tumor-infiltrating immune cells are associated with increased relapse-free survival in patients with estrogen receptor-negative, progesterone receptor-negative, triple-negative, HER2-positive, or basal-like breast cancer.6 Protein levels of VISTA are also increased in peripheral blood mononuclear cells (PBMCs) isolated from patients with myasthenia gravis.7 Cayman’s VISTA Rabbit Monoclonal Antibody (Clone RM503) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. ElTanbouly, M.A., Croteau, W., Noelle, R.J., et al. VISTA: A novel immunotherapy target for normalizing innate and adaptive immunity. Semin. Immunol. 42, 101308 (2019).

    2. Nowak, E.C., Lines, J.L., Varn, F.S., et al. Immunoregulatory functions of VISTA. Immunol. Rev. 276(1), 66-79 (2017).

    3. Ni, L., and Dong, C. New checkpoints in cancer immunotherapy. Immunol. Rev. 276(1), 52-65 (2017).

    4. Wang, G., Tai, R., Wu, Y., et al. The expression and immunoregulation of immune checkpoint molecule VISTA in autoimmune diseases and cancers. Cytokine Growth Factor Rev. 52, 1-14 (2020).

    5. Lines, J.L., Pantazi, E., Mak, J., et al. VISTA is an immune checkpoint molecule for human T cells. Cancer Res. 74(7), 1924-1932 (2014).

    6. Zong, L., Mo, S., Yu, S., et al. Expression of the immune checkpoint VISTA in breast cancer. Cancer Immunol. Immunother. 69(8), 1437-1446 (2020).

    7. Ren, Y., Qi, F., Li, J., et al. Expression and correlation analysis of VISTA in peripheral blood mononuclear cells of myasthenia gravis patients. Int. Immunopharmacol. 148:114096, (2025).