For immunochemical detection of DNA-PKcs
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DNA-PKcs Rabbit Monoclonal Antibody (Clone RM505)

Item No. 42856

Product Insert (PDF)
Technical Information
Synonyms
  • DNA-dependent Protein Kinase Catalytic Subunit
  • DNA-PK Catalytic Subunit
  • p460
Immunogen
A peptide corresponding to the C-terminus of human DNA-PKcs
Clone Designation
RM505
Formulation (Request formulation change)
100 µl of protein A-affinity purified antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

    Add
    Cayman Chemical
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    Product Description

    DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is a member of the PI3K-related kinase (PIKK) family.1,2,3 It is composed of an N-terminal domain, which consists of two domains of HEAT repeats, a FAT homology domain, a FKBP12-rapamycin-binding (FRB) domain, a kinase domain, a PIKK regulatory domain, and a C-terminal FAT domain (FATC). DNA-PKcs localizes to the nucleus, cytoplasm, plasma membrane, cytoskeleton, and lipid rafts.2,4 It is involved in DNA double-strand break (DSB) repair through non-homologous end-joining (NHEJ) and homologous recombination (HR) pathways and contains numerous phosphorylation sites that modify its activity, such as Ser2056, which must be autophosphorylated for NHEJ to occur in response to DSBs.1,2,3 DNA-PKcs also plays a role in mitosis, telomere maintenance, transcriptional regulation, innate and adaptive immunity, and senescence, among many other processes. DNA-PKcs interacts with Ku70/Ku80 to form the DNA-PK holoenzyme, which acts on various substrates, including histone H2AX, heat shock protein 90 (Hsp90), and Artemis.2,3 High expression or increased activity of DNA-PKcs is associated with metastasis, poor prognosis, and radio- and chemoresistance in various cancers.2 Cayman's DNA-PKcs Rabbit Monoclonal Antibody (Clone RM505) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Mohiuddin, I.S., and Kang, M.H. DNA-PK as an emerging therapeutic target in cancer. Front. Oncol. 9, 635 (2019).

    2. Dylgjeri, E., and Knudsen, K.E. DNA-PKcs: A targetable protumorigenic protein kinase. Cancer Res. 82(4), 523-533 (2022).

    3. Wu, J., Song, L., Lu, M., et al. The multifaceted functions of DNA-PKcs: Implications for the therapy of human diseases. MedComm (2020) 5(7), e613 (2024).

    4. Carter, T., Vancurová, I., Sun, I., et al. A DNA-activated protein kinase from HeLa cell nuclei. Mol. Cell Biol. 10(12), 6460-6471 (1990).