Histone H3^K36M Rabbit Monoclonal Antibody - Biotinylated (Clone RM193)

Histone 3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.¹ It is a globular protein that contains a histone fold domain with a C-terminal α-helix that facilitates nucleosome interactions and chromatin compaction, as well as an unstructured N-terminal tail that extends outside of the nucleosome core, both of which are subject to various post-translational modifications (PTMs), including ubiquitination, acetylation, methylation, and phosphorylation.^1,2,3 Histone H3 has a key role in transcriptional regulation.⁴ A lysine-to-methionine mutation at residue 36 in histone H3.3 (H3.3^K36M) is associated with chondroblastomas.⁵ Cayman’s Histone H3^K36M Rabbit Monoclonal Antibody - Biotinylated (Clone RM193) can be used for chromatin immunoprecipitation (ChIP), ELISA, immunohistochemistry (IHC), and Western blot (WB) applications.

WARNING This product is not for human or veterinary use.

1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

2. Wyrick, J.J., and Parra, M.A. The role of histone H2A and H2B post-translational modifications in transcription: A genomic perspective. Biochim. Biophys. Acta 1789(1), 37-44 (2009).

3. Wang, C.-Y., Hua, C.-Y., Hsu, H.-E., et al. The C-terminus of histone H2B is involved in chromatin compaction specifically at telomeres, independently of its monoubiquitylation at lysine 123. PLoS One 6(7), e22209 (2011).

4. Bhaumik, S.R., Smith, E., and Shilatifard, A. Covalent modifications of histones during development and disease pathogenesis. Nat. Struct. Mol. Biol. 14(11), 1008-1016 (2007).

5. Fang, D., Gan, H., Lee, J.-H., et al. The histone H3.3K36M mutation reprograms the epigenome of chondroblastomas. Science 352(6291), 1344-1348 (2016).