For immunochemical detection of histone H3.3G34V
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Histone H3.3G34V Rabbit Monoclonal Antibody - Biotinylated (Clone RM307)

Item No. 42870

Product Insert (PDF)
Technical Information
Immunogen
A peptide corresponding to histone H3.3G34V
Clone Designation
RM307
Formulation (Request formulation change)
50 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, IHC, WB
Cross Reactivity
(+) Histone H3.3G34V(-) Wild-type histone H3.3
Species Reactivity
(+) Independent
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    50 µl

    $411

    Add
    Cayman Chemical
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    Product Description

    Histone H3 is a nuclear protein and component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs). Metazoans have three histone variants: canonical histone H3.1, DNA replication-independent histone H3.3, and the centromeric variant CENP-A.2 Histone H3.3 is incorporated into chromatin in a DNA replication-independent manner at sites of active transcription.2,3,4 A missense mutation replacing guanine with adenine in H3F3A, the gene encoding histone H3.3, leads to a glycine-to-valine substitution, histone H3.3G34V, which is associated with glioblastoma multiforme in children and young adults.5 Cayman's Histone H3.3G34V Rabbit Monoclonal Antibody (Clone RM307) - Biotinylated can be used for ELISA, immunohistochemistry (IHC), and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Elsaesser, S.J., Goldberg, A.D., and Allis, C.D. New functions for an old variant: No substitute for histone H3.3. Curr. Opin. Genet. Dev. 20(2), 110-117 (2010).

    3. Bjerke, L., Mackay, A., Nandhabalan, M., et al. Histone H3.3 mutations drive pediatric glioblastoma through upregulation of MYCN. Cancer Discov. 3(5), 512-519 (2013).

    4. Tagami, H., Ray-Gallett, D., Almouzni, G., et al. Histone H3.1 and H3.3 complexes mediate nucleosome assembly pathways dependent or independent of DNA synthesis. Cell 116(1), 51-61 (2004).

    5. Schwartzentruber, J., Korshunov, A., Liu, X.-Y., et al. Driver mutations in histone H3.3 and chromatin remodelling genes in paediatric glioblastoma. Nature 482(7384), 226-231 (2012).