For immunochemical detection of 5-mC
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5-Methylcytosine DNA Matched Antibody Pair (Clones RM231/RM476)

Item No. 42873

Product Insert (PDF)
Technical Information
Synonyms
  • 5-mC
  • m5C
  • NSC 137776
Immunogen
BSA-conjugated 5-mC
Clone Designation
RM/231/RM476
Formulation (Request formulation change)
Protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol and 0.09% sodium azide and PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Applications
Sandwich ELISAs, other sandwich assays
Cross Reactivity
(+) 5-mC(-) Non-methylated cytosine(-) 5-hmC(-) 5-caC(-) 5-fC
Species Reactivity
(Independent) Species
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    1 ea

    $702

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    Product Description

    5-Methylcytosine (5-mC) is an epigenetic modification formed from methylation of cytosine by DNA methyltransferases (DNMTs).1 5-mC is an abundant DNA modification that is enriched at CpG dinucleotides. It can be successively oxidized by ten-eleven translocation (TET) dioxygenases to form 5-hydroxymethylcytosine (5-hmC), 5-formylcytosine (5-fC), and 5-carboxylcytosine (5-caC). 5-mC is associated with reduced gene transcription of DNA but is also found in the tRNA, mRNA, and rRNA from bacteria, archaea, and eukaryotes.1,2 Levels of 5-mC on CpG islands in the genes encoding p16, thrombospondin-1 (TSP-1), and methylated in tumor 1 (MINT1) are increased in tumors compared with adjacent normal tissue in patients with renal cell carcinoma.3 Cayman’s 5-Methylcytosine and Adenine-Guanine - Biotinylated Matched Rabbit Monoclonal Antibody Pair (Clones RM231/RM476) contains 5-Methylcytosine Rabbit Monoclonal Antibody (Clone RM231) (Item No. 20722) as a capture antibody and Adenine-Guanine Rabbit Monoclonal Antibody - Biotinylated (Clone RM476) as a detection antibody. This antibody pair can be used for sandwich ELISAs or other sandwich assays for the specific detection of 5-mC in ssDNA.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Breiling, A., and Lyko, F. Epigenetic regulatory functions of DNA modifications: 5-Methylcytosine and beyond. Epigenetics Chromatin 8, 24 (2015).

    2. Edelheit, S., Schwartz, S., Mumbach, M.R., et al. Transcriptome-wide mapping of 5-methylcytidine RNA modifications in bacteria, archaea, and yeast reveals m5C within archaeal mRNAs. PLoS Genetics 9(6), e1003602 (2013).

    3. Arai, E., Kanai, Y., Ushijima, S., et al. Regional DNA hypermethylation and DNA methyltransferase (DNMT) 1 protein overexpression in both renal tumors and corresponding nontumorous renal tissues. Int. J. Cancer 119(2), 288-296 (2006).