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The Naveni® BOND RX Atto647N is an in situ proximity ligation assay kit using BOND RX/RXm Fully Automated Research Stainer. The kit has been validated on FFPE human tissue samples.
The kit is based on the Naveni® in situ proximity ligation technology with an automated workfflow. It uses two Navenibodies linked to proprietary oligo arms. The to Navenibodies included in the kit will bind primary antibodies of mouse and rabbit origin. When Navenbodies are in close proximity, a strong and distinct signal is generated. Read more about Naveni technology.
Included in the kit:
• Anti-mouse Navenibody
• Anti-rabbit Navenibody
• Buffers for blocking and dilutions, and all detection reagents
• Reagents sufficient for 30 FFPE tissue slides, including dead volumes
Not included in the kit:
• Mouse Anitbody 1
• Rabbit Anitbody 1
• Leica Biosystems BOND titration kit (6 ml)
• Leica Biosystems BOND Research Detection System or Research Detection System 2
• Leica Biosystems BOND Universal Covertiles
• Leica Biosystems Slide and Reagent Trays
• Leica Biosystems Slide Labels and Printer Ribbon
• Leica Biosystems BOND Dewax Solution
• Leica Biosystems BOND Epitope Retrieval Solution 1 and 2
• Leica Biosystems BOND Wash Solution 10X Concentrate
• Leica Biosystems BOND Aspirating Probe Cleaning Kit
• Mounting medium for fluorescent dyes
• Coverslip for microscope slides
• DAPI (diluted in PBS to 1 µg/ml)
• Deionized water
• Reagent grade alcohol
• TBS-T
Software requirements to run NaveniFlex BOND RX
• BOND RX v7.0 software
• BXD 40
WARNING This product is not for human or veterinary use.