A colorimetric protease substrate
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Cbz-Gly-Pro-Arg-pNA (acetate)

Item No. 42990

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-[(phenylmethoxy)carbonyl]glycyl-L-prolyl-N-(4-nitrophenyl)-L-argininamide, acetate
Synonyms
  • Cbz-GPR-pNA
  • Cbz-GPR-p-nitroanilide
  • Protease Chromogenic Substrate
Molecular Formula
C27H34N8O7 • XC2H4O2
Formula Weight
582.6
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: Sparingly soluble: 1-10 mg/mlEthanol: Sparingly soluble: 1-10 mg/ml
SMILES
O=C([C@@H]1CCCN1C(CNC(OCC2=CC=CC=C2)=O)=O)N[C@H](C(NC3=CC=C(C=C3)[N+]([O-])=O)=O)CCCNC(N)=N.CC(O)=O
InChi Code
InChI=1S/C27H34N8O7.C2H4O2/c28-26(29)30-14-4-8-21(24(37)32-19-10-12-20(13-11-19)35(40)41)33-25(38)22-9-5-15-34(22)23(36)16-31-27(39)42-17-18-6-2-1-3-7-18;1-2(3)4/h1-3,6-7,10-13,21-22H,4-5,8-9,14-17H2,(H,31,39)(H,32,37)(H,33,38)(H4,28,29,30);1H3,(H,3,4)/t21-,22-;/m0./s1
InChi Key
FPMAXHHYPVIJMC-VROPFNGYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $37

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    5 mg

    $164

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    10 mg

    $291

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    25 mg

    $363

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    Product Description

    Cbz-Gly-Pro-Arg-pNA is a colorimetric substrate for proteases, including thrombin and mast cell tryptase.1,2 Proteases preferentially bind to and cleave the Gly-Pro-Arg (GPR) peptide sequence to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of protease activity.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hogg, P.J., and Jackson, C.M. Formation of a ternary complex between thrombin, fibrin monomer, and heparin influences the action of thrombin on its substrates. The Journal of Biological Chemisty 265(1), 248-255 (1990).

    2. Harvima, I.T., Harvima, R.J., Eloranta, T.O., et al. The allosteric effect of salt on human mast cell tryptase. Biochim. Biophys. Acta 956(2), 133-139 (1988).