For immunochemical detection of Prx6
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Peroxiredoxin-6 Monoclonal Antibody (Clone 03)

Item No. 43212

Product Insert (PDF)
Technical Information
Synonyms
  • Acidic Calcium-independent Phospholipase A2
  • aiPLA2
  • 1-Cys Peroxiredoxin
  • Glutathione-dependent Peroxiredoxin
  • LPCAT5
  • Non-selenium Glutathione Peroxidase
  • NSGPx
  • Prx6
Immunogen
Recombinant human Prx6
Clone Designation
03
Formulation (Request formulation change)
100 µl of protein A-purified monoclonal antibody
Storage Buffer
0.2 μm filtered solution in PBS, with 10% trehalose, pH 7.0
Host
Mouse
Isotype
IgG1
Applications
ELISA, IHC-P, IP
Cross Reactivity
(+) Prx6(-) E. coli cell lysate
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µl

    $296

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    Product Description

    Peroxiredoxin-6 (Prx6) is a 1-Cys-containing Prx that is involved in the maintenance of cellular thiol redox homeostasis and phospholipid metabolism.1 It exists as a homodimer and is composed of a thioredoxin fold-containing catalytic domain, which contains a peroxidatic cysteine (Cp), and a C-terminal arm.2 Unlike 2-Cys-containing Prxs, Prx6 does not contain a resolving cysteine (Cr) and uses glutathione (GSH) instead. Prx6 is ubiquitously expressed and localizes to the cytosol, nucleus, mitochondria, and plasma membrane.1,3 It has three enzymatic activities: glutathione peroxidase (GPX), acidic calcium-independent phospholipase A2 (aiPLA2), and lysophosphatidylcholine acyltransferase (LPCAT).1,4,5 The LPCAT activity, which selectively uses palmitoyl-CoA as a substrate, is coupled to the aiPLA2 activity and is selective for lysophosphatidylcholine over lysophosphatidylethanolamine, lysophosphatidylglycerol, lysophosphatidylinositol, and lysophosphatidylserine.5 These activities, coupled with the GPX activity, provide Prx6 the ability to repair oxidized cell membranes.1 Prx6 is also involved in the activation of NADPH oxidase 2 (NOX2). Tumor levels of Prx6 are increased in patients with non-small cell lung cancer (NSCLC) and serum levels of Prx6 are decreased in patients with asthma.6 Cayman's Peroxiredoxin-6 Monoclonal Antibody (Clone 03) can be used for ELISA, immunohistochemistry-paraffin (IHC-P), and immunoprecipitation (IP) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Arevalo, J.A., and Vázquez-Medina, J.P. The role of peroxiredoxin 6 in cell signaling. Antioxidants (Basel) 7(12), 172 (2018).

    2. Choi, H.J., Kang, S.W., Yang, C.H., et al. Crystal structure of a novel human peroxidase enzyme at 2.0 A resolution. Nat. Struct. Biol. 5(5), 400-406 (1998).

    3. Dammeyer, P., and Arnér, E.S. Human Protein Atlas of redox systems - What can be learnt? Biochim. Biophys. Acta 1810(1), 111-138 (2011).

    4. Chen, J.W., Dodia, C., Feinstein, S.I., et al. 1-Cys peroxiredoxin, a bifunctional enzyme with glutathione peroxidase and phospholipase A2 activities. The Journal of Biological Chemisty 275(37), 28421-28427 (2000).

    5. Fisher, A.B., Dodia, C., Sorokina, E.M., et al. A novel lysophosphatidylcholine acyl transferase activity is expressed by peroxiredoxin 6. J. Lipid Res. 57(4), 587-596 (2016).

    6. Jia, W., Dong, C., and Li, B. Anti-oxidant and pro-oxidant effects of peroxiredoxin 6: A potential target in respiratory diseases. Cells 12(1), 181 (2023).