BSA-Decanoate Saturated Fatty Acid Complex (1 mM)

BSA-Decanoate Saturated Fatty Acid Complex (1 mM) is composed of decanoic acid (Item No. 20838 ) and fatty-acid free bovine serum albumin (BSA) at an approximately 6:1 molar ratio of decanoate:BSA. It can be used for efficient fatty acid delivery to cells in culture for the purpose of monitoring lipid metabolism and inflammatory signaling pathways and mimicking a ketogenic diet.^1,2,3 Cayman’s BSA-Decanoate Saturated Fatty Acid Complex (1 mM) is suitable for use in short-term cell culture applications (acute treatment to 18 hours); however, for long-term applications (25+ hours) the product should be filter-sterilized using a 0.2 µm filter and sterile receptacle, which will not affect its performance. For best results, it is recommended that this product be used in conjunction with Cayman’s BSA Control for BSA-Fatty Acid Complexes (1 mM) (Item No. 34932 ), prepared with fatty acid-free BSA.

WARNING This product is not for human or veterinary use.

1. Alsabeeh, N., Chausse, B., Kakimoto, P.A., et al. Cell culture models of fatty acid overload: Problems and solutions. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1863(2), 143-151 (2018).

2. Sato, K., Cho, Y., Tachibana, S., et al. Impairment of VLDL secretion by medium-chain fatty acids in chicken primary hepatocytes is affected by the chain length. J. Nutr. 135(7), 1636-1641 (2005).

3. Kanabus, M., Fassone, E., Hughes, S.D., et al. The pleiotropic effects of decanoic acid treatment on mitochondrial function in fibroblasts from patients with complex I deficient Leigh syndrome. J. Inherit. Metab. Dis. 39(3), 415-426 (2016).