Host: HEK293 cells • AA: 31-1,012 • Tag: C-terminal His • MW: 109 kDa
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Contactin-2 (human, recombinant)

Item No. 43686

Product Insert (PDF)
Technical Information
Synonyms
  • Axonal Glycoprotein TAG-1
  • Axonin-1
  • CNTN2
  • TAG-1
  • TAX-1
  • Transient Axonal Glycoprotein 1
Purity
≥95% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU per ug of the protein as determined by the LAL method
Source
Recombinant human C-terminal His-tagged contactin-2 expressed in HEK293 cells
Amino Acids
1-1,012
MW
109 kDa
Formulation (Request formulation change)
Lyophilized from sterile 100 mM glycine, 10 mM sodium chloride, 50 mM Tris, pH 7.5
UniProt Accession №
Q02246
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    100 µg

    $328

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    1 mg

    $2,131

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    Product Description

    Contactin-2 is a neural cell adhesion molecule and member of the immunoglobulin (Ig) superfamily.1 It is composed of six Ig-like repeats, four fibronectin type III-like domains, and a hydrophobic C-terminal sequence that contains a glycosylphosphatidylinositol (GPI) anchor site. Contactin-2 localizes to the cell surface but also exists as a soluble form, which is produced via proteolytic cleavage by β-secretase 1 (BACE1).2 It is transiently expressed in neurons during embryonic development and in the brain and spinal cord in adult tissues.1,3 It is involved in axonal growth, guidance, and fasciculation and myelinated fiber formation and maintenance.1 Contactin-2 interacts with several partners, notably contactin-associated protein-like 2 (CASPR2) and juxtaparanodal potassium channels. Brain levels of contactin-2 are decreased in patients with Alzheimer’s disease.2 A frameshift mutation in the gene encoding contactin-2, CNTN2, is associated with epilepsy.4 Cayman’s Contactin-2 (human, recombinant) protein consists of 993 amino acids, has a calculated molecular weight of 109 kDa, and a predicted N-terminus of Ser31 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 140 kDa due to glycosylation.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Masuda, T. Contactin-2/TAG-1, active on the front line for three decades. Cell Adh. Migr. 11(5-6), 524-531 (2017).

    2. Gautam, V., D'Avanzo, C., Hebisch, M., et al. BACE1 activity regulates cell surface contactin-2 levels. Mol. Neurodegener. 9, 4 (2014).

    3. Kamei, Y., Takeda, Y., Teramoto, K., et al. Human NB-2 of the contactin subgroup molecules: Chromosomal localization of the gene (CNTN5) and distinct expression pattern from other subgroup members. Genomics 69(1), 113-119 (2000).

    4. Chen, W., Chen, F., Shen, Y., et al. Case report: A case of epileptic disorder associated with a novel CNTN2 frameshift variant in homozygosity due to maternal uniparental disomy. Front. Genet. 12, 743833 (2021).