Host: HEK293 cells • AA: 20-1,002 • Tag: C-terminal human IgG1 Fc • MW: 134.5 kDa
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Contactin-3 (human, recombinant)

Item No. 43687

Product Insert (PDF)
Technical Information
Synonyms
  • BIG-1
  • Brain-derived Immunoglobulin Superfamily Protein 1
  • CNTN3
  • PANG
  • PCS
  • Plasmacytoma-associated Neuronal Glycoprotein
Purity
≥90% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/µg determined by the LAL endotoxin assay
Amino Acids
20-1,002
MW
134.5 kDa
Formulation (Request formulation change)
Lyophilized from sterile 100 mM glycine, 10 mM sodium chloride, 50 mM Tris, pH 7.5
Host
HEK293 cells
UniProt Accession №
Q9P232
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $328

    Add
    Cayman Chemical
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    Product Description

    Contactin-3 is a neural cell adhesion molecule and member of the immunoglobulin (Ig) superfamily.1 It is composed of six Ig-like repeats, four fibronectin type III-like domains, and a hydrophobic C-terminal sequence that contains a glycosylphosphatidylinositol (GPI) anchor site. Contactin-3 is primarily expressed in the frontal lobe, occipital lobe, cerebellum, and amygdala in the brain where it interacts with amyloid precursor protein (APP), amyloid-β precursor-like protein 1 (APLP-1), and protein tyrosine phosphatase γ (PTPγ).2,3 Expression of CNTN3, the gene encoding contactin-3, is decreased in cortical tubers isolated from patients with tuberous sclerosis complex (TSC), a condition characterized by increased incidence of epilepsy and intellectual impairment.4 Low expression levels of CNTN3 are associated with decreased overall survival and poor prognosis in patients with glioblastoma multiforme.5 Cayman's Contactin-3 (human, recombinant) protein is a disulfide-linked homodimer. This protein is a disulfide-linked homodimer. The reduced monomer, composed of contactin-3 (amino acids 20-1,002) fused to IgG1 Fc at its C-terminus, consists of 1,221 amino acids, has a calculated molecular weight of 134.5 kDa, and a predicted N-terminus of Gly20 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 160-170 kDa due to glycosylation.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Shimoda, Y., and Watanabe, K. Contactins: Emerging key roles in the development and function of the nervous system. Cell Adh. Migr. 3(1), 64-70 (2009).

    2. Kamei, Y., Takeda, Y., Teramoto, K., et al. Human NB-2 of the contactin subgroup molecules: Chromosomal localization of the gene (CNTN5) and distinct expression pattern from other subgroup members. Genomics 69(1), 113-119 (2000).

    3. Nikolaienko, R.M., Hammel, M., Dubreuil, V., et al. Structural basis for interactions between contactin family members and protein-tyrosine phosphatase receptor type G in neural tissues. The Journal of Biological Chemisty 291(41), 21335-21349 (2016).

    4. Korotkov, A., Luinenburg, M.J., Romagnolo, A., et al. Down-regulation of the brain-specific cell-adhesion molecule contactin-3 in tuberous sclerosis complex during the early postnatal period. J. Neurodev. Disord. 14(1), 8 (2022).

    5. Zhu, Y.-F., Guo, Y.-B., Zhang, H.-Y., et al. Prognostic significance of contactin 3 expression and associated genes in glioblastoma multiforme. Oncol. Lett. 18(2), 1863-1871 (2019).