Host: E. coli • AA: Met1; 24-303 • Tag: C-terminal His • MW: 35 kDa
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Caspase-7 (human, recombinant)

Item No. 43692

Product Insert (PDF)
Technical Information
Synonyms
  • CASP-7
  • Apoptotic Protease Mch-3
  • CMH-1
  • ICE-like Apoptotic Protease 3
  • ICE-LAP3
Purity
≥90% estimated by SDS-PAGE
Source
Recombinant human C-terminal His-tagged caspase-7 expressed in E. coli
Amino Acids
Met1; 24-303
MW
35 kDa
Formulation (Request formulation change)
Lyophilized from sterile 20 mM HEPES, pH 7.5, with 100 mM sodium chloride, 1 mM EDTA, 0.10% sucrose, and 0.1% CHAPS
Host
E. coli
UniProt Accession №
P55210
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    20 µg

    $344

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    Product Description

    Caspase-7 is a cysteinyl aspartic protease and a member of the caspase family of proteases.1 It is expressed as a proprotein that upon pro-apoptotic signaling is cleaved into two subunits, p20 and p11, which form an active homodimer that recognizes and cleaves proteins containing the amino acid sequence DEVD.1,2 Caspase-7 is ubiquitously expressed but is found at low levels in the brain and localizes to the cytoplasm.3 It is an effector caspase and targets the same protein substrates as caspase-3 but is less effective at inducing apoptosis.4,5 However, it can induce apoptosis in response to specific signaling, such as granzyme B-induced poly(ADP-ribose) polymerase (PARP) cleavage.3,4 Caspase-7 cleaves and activates acid sphingomyelinase to repair gasdermin D (GSDMD) and perforin pores in the plasma membrane and prevents non-apoptotic forms of cell death.6 Increased intratumoral levels of cleaved caspase-7 are positively associated with increased overall survival in patients with breast cancer.7 Cayman’s Caspase-7 (human, recombinant) protein was synthesized from a DNA sequence encoding the mature form of human caspase-7 (Ala24-Gln303) with an N-terminal translation-initiating methionine (Met1). The expressed protein consists of 313 amino acids, has a calculated molecular weight of 35 kDa, and a predicted N-terminus of Met1.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lamkanfi, M., and Kanneganti, T.D. Caspase-7: A protease involved in apoptosis and inflammation. Int. J. Biochem. Cell Biol. 42(1), 21-24 (2010).

    2. Talanian, R.V., Quinlan, C., Trautz, S., et al. Substrate specificities of caspase family proteases. The Journal of Biological Chemisty 272(15), 9677-9682 (1997).

    3. Cohen, G.M. Caspases: The executioners of apoptosis. Biochem. J. 326(Pt 1), 1-16 (1997).

    4. Slee, E.A., Adrain, C., and Martin, S.J. Executioner caspase-3, -6, and -7 perform distinct, non-redundant roles during the demolition phase of apoptosis. The Journal of Biological Chemisty 276(10), 7320-7326 (2001).

    5. Walsh, J.G., Cullen, S.P., Sheridan, C., et al. Executioner caspase-3 and caspase-7 are functionally distinct proteases. Proc. Natl. Acad. Sci. USA 105(35), 12815-12819 (2008).

    6. Nozaki, K., Maltez, V.I., Rayamajhi, M., et al. Caspase-7 activates ASM to repair gasdermin and perforin pores. Nature 606(7916), 960-967 (2022).

    7. Lindner, A.U., Lucantoni, F., Varešlija, D., et al. Low cleaved caspase-7 levels indicate unfavourable outcome across all breast cancers. J. Mol. Med. (Berl) 96(10), 1025-1037 (2018).