Host: HEK293 cells • AA: 62-199 • Tag: C-terminal His • MW: 17.4 kDa
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

CD69 Extracellular Domain (human, recombinant)

Item No. 43694

Product Insert (PDF)
Technical Information
Synonyms
  • Activation Inducer Molecule
  • AIM
  • CLEC2C
  • Cluster of Differentiation 69
  • EA1
  • Early Activation Antigen CD69
  • Early T-cell Activation Antigen p60
  • C-type Lectin Domain Family 2 Member C
  • Leukocyte Surface Antigen Leu-23
  • MLR-3
Purity
≥90% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/μg, determined by the LAL endotoxin assay
Source
Recombinant human C-terminal His-tagged CD69 extracellular domain expressed in HEK293 cells
Amino Acids
62-199
MW
17.4 kDa
Formulation (Request formulation change)
Lyophilized from sterile PBS, pH 7.4
UniProt Accession №
Q07108
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    50 µg

    $218

    Add

    500 µg

    $1,307

    Add
    Lipid Resource Center
    Discover Products & Resources for Lipid Research
    • High-purity lipid standards
    • Lipid roles in biology
    • Lipids in health & disease
    • Lipids for pharmaceutical development
    • Protocols, advice, & resources
    EXPLORE NOW
    Product Description

    CD69 is a membrane-bound type II C-lectin receptor and early activation marker of leukocytes.1,2,3 It is composed of an extracellular C-type lectin domain, a neck region, a transmembrane domain, and a cytoplasmic tail and exists as a homodimer.3 CD69 is expressed in activated hematopoietic cells, such as T cells, B cells, and platelets, as well as neutrophils, eosinophils, and epidermal Langerhans cells.4 It is involved in many biological processes, including T cell differentiation, cell migration, immune checkpoint regulation, and metabolic reprogramming, and acts as a receptor for various ligands, such as galectin-1 (Gal-1) and oxidized low-density lipoprotein (oxLDL).1,2,3 CD69 also acts as an agonist of sphingosine-1-phosphate receptor 1 (S1P1).2,3,5 Increased intratumoral levels of CD69 are associated with poor prognosis in patients with hepatocellular carcinoma.6 Cayman’s CD69 Extracellular Domain (human, recombinant) protein is a disulfide-linked homodimer. The reduced monomer, composed of CD69 (amino acids 62-199) fused to a signal peptide and His-tag at the N- and C-terminus, respectively, consists of 149 amino acids, has a calculated molecular weight of 17.4 kDa, and a predicted N-terminus of Ser62 after signal peptide cleavage. The homodimer migrates at approximately 26.99 and 23.11 kDa by SDS-PAGE under reducing conditions.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Jiménez-Fernández, M., de la Fuente, H., Martín, P., et al. Unraveling CD69 signaling pathways, ligands and laterally associated molecules. EXCLI J. 22, 334-351 (2023).

    2. Cibrián, D., and Sánchez-Madrid, F. CD69: From activation marker to metabolic gatekeeper. Eur. J. Immunol. 47(6), 946-953 (2017).

    3. Radulovic, K., and Niess, J.H. CD69 is the crucial regulator of intestinal inflammation: A new target molecule for IBD treatment? J. Immunol. Res. 497056 (2015).

    4. Hamann, J., Fiebig, H., and Strauss, M. Expression cloning of the early activation antigen CD69, a type II integral membrane protein with a C-type lectin domain. J. Immunol. 150(11), 4920-4927 (1993).

    5. Chen, H., Qin, Y., Chou, M., et al. Transmembrane protein CD69 acts as an S1PR1 agonist. bioRxiv [Preprint] (2023).

    6. Tang, K., Li, X., Mo, J., et al. CD69 serves as a potential diagnostic and prognostic biomarker for hepatocellular carcinoma. Sci. Rep. 13(1), 7452 (2023).