CD69 Extracellular Domain (human, recombinant)

CD69 is a membrane-bound type II C-lectin receptor and early activation marker of leukocytes.^1,2,3 It is composed of an extracellular C-type lectin domain, a neck region, a transmembrane domain, and a cytoplasmic tail and exists as a homodimer.³ CD69 is expressed in activated hematopoietic cells, such as T cells, B cells, and platelets, as well as neutrophils, eosinophils, and epidermal Langerhans cells.⁴ It is involved in many biological processes, including T cell differentiation, cell migration, immune checkpoint regulation, and metabolic reprogramming, and acts as a receptor for various ligands, such as galectin-1 (Gal-1) and oxidized low-density lipoprotein (oxLDL).^1,2,3 CD69 also acts as an agonist of sphingosine-1-phosphate receptor 1 (S1P₁).^2,3,5 Increased intratumoral levels of CD69 are associated with poor prognosis in patients with hepatocellular carcinoma.⁶ Cayman’s CD69 Extracellular Domain (human, recombinant) protein is a disulfide-linked homodimer. The reduced monomer, composed of CD69 (amino acids 62-199) fused to a signal peptide and His-tag at the N- and C-terminus, respectively, consists of 149 amino acids, has a calculated molecular weight of 17.4 kDa, and a predicted N-terminus of Ser62 after signal peptide cleavage. The homodimer migrates at approximately 26.99 and 23.11 kDa by SDS-PAGE under reducing conditions.

WARNING This product is not for human or veterinary use.

1. Jiménez-Fernández, M., de la Fuente, H., Martín, P., et al. Unraveling CD69 signaling pathways, ligands and laterally associated molecules. EXCLI J. 22, 334-351 (2023).

2. Cibrián, D., and Sánchez-Madrid, F. CD69: From activation marker to metabolic gatekeeper. Eur. J. Immunol. 47(6), 946-953 (2017).

3. Radulovic, K., and Niess, J.H. CD69 is the crucial regulator of intestinal inflammation: A new target molecule for IBD treatment? J. Immunol. Res. 497056 (2015).

4. Hamann, J., Fiebig, H., and Strauss, M. Expression cloning of the early activation antigen CD69, a type II integral membrane protein with a C-type lectin domain. J. Immunol. 150(11), 4920-4927 (1993).

5. Chen, H., Qin, Y., Chou, M., et al. Transmembrane protein CD69 acts as an S1PR1 agonist. bioRxiv [Preprint] (2023).

6. Tang, K., Li, X., Mo, J., et al. CD69 serves as a potential diagnostic and prognostic biomarker for hepatocellular carcinoma. Sci. Rep. 13(1), 7452 (2023).