Prominin-1/CD133 Splice Variant s7 EC2 Domain (rat, recombinant)

CD133, also known as prominin-1, is a five-transmembrane domain glycoprotein and stem cell marker.¹ It is composed of an N-terminal extracellular domain (EC1) and two extracellular loops (EC2 and EC3), which are linked via two intracellular loops (IC1 and IC2), and a C-terminal cytoplasmic domain (IC3). CD133 localizes to membrane protrusions and undergoes extensive alternative splicing, where each splice variant is expressed in a tissue-dependent manner.^1,2,3 In addition to its expression in stem cells, CD133 is also expressed in some differentiated epithelial cells, such as in the proximal tubules of the kidneys, epididymal tract, and various glands, and differentiated nonepithelial cells, including photoreceptor cells.¹ It is involved in regulating membrane architecture of microvilli and cilia, stem cell transit, autophagy, interactions with lipid rafts, and intercellular communication, among other processes. CD133 is commonly used as an antigen to isolate stem cells, including cancer stem cells, from biological samples.^1,4 Mutations in PROM1, the gene encoding CD133, are associated with macular degeneration.⁵ Cayman’s Prominin-1/CD133 Splice Variant s7 EC2 Domain (rat, recombinant) protein consists of 274 amino acids and has a calculated molecular weight of 30.9 kDa. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is approximately 47 to 57 kDa.

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1. Pleskač, P., Fargeas, C.A., Veselska, R., et al. Emerging roles of prominin-1 (CD133) in the dynamics of plasma membrane architecture and cell signaling pathways in health and disease. Cell. Mol. Biol. Lett. 29(1), 41 (2024).

2. Fargeas, C.A., Huttner, W.B., and Corbeil, D. Nomenclature of prominin-1 (CD133) splice variants - an update. Tissue Antigens 69(6), 602-606 (2007).

3. Shmelkov, S.V., Jun, L., St Clair, R., et al. Alternative promoters regulate transcription of the gene that encodes stem cell surface protein AC133. Blood 103(6), 2055-2061 (2004).

4. Haraguchi, N., Utsunomiya, T., Inoue, H., et al. Characterization of a side population of cancer cells from human gastrointestinal system. Stem Cells (2005).

5. Yang, Z., Chen, Y., Lillo, C., et al. Mutant prominin 1 found in patients with macular degeneration disrupts photoreceptor disk morphogenesis in mice. J. Clin. Invest. 118(8), 2908-2916 (2008).