Host: Insect cells • AA: 1-342 (full length) • Tag: N-terminal GST • MW: 68 kDa
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WDR77 (human, recombinant)

Item No. 43756

Product Insert (PDF)
Technical Information
Synonyms
  • Androgen Receptor Cofactor p44
  • MEP-50
  • Methylosome Protein 50
  • Methylosome Protein WDR77
  • WD Repeat-containing protein 77
Purity
≥90% estimated by SDS-PAGE
Source
Recombinant human N-terminal GST-tagged WDR77 expressed in insect cells
Amino Acids
1-342
MW
68 kDa
Formulation (Request formulation change)
Sterile 50 mM Tris-HCl, pH 7.5, with 50-300 mM sodium chloride, 10 mM glutathione, 0.1 mM EDTA, 0.25-1.0 mM DTT, 0-0.1 mM PMSF, and 10-25% glycerol
UniProt Accession №
Q9BQA1
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    20 µg

    $284

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    100 µg

    $923

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    1 mg

    $4,257

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    Cayman Chemical
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    Product Description

    Methylosome protein WDR77, also known as methylosome protein 50 (MEP50) and androgen receptor cofactor p44, is an obligate cofactor of protein arginine methyltransferase 5 (PRMT5) and androgen receptor coactivator.1,2 It contains 342 amino acid residues and six putative WD-40 repeats that act as scaffolds for protein-protein interactions.3 WDR77 is ubiquitously expressed and localizes to both the cytoplasm and nucleus.1,2 It binds to PRMT5 to stabilize and increase its activity, as well as recruits substrates to the PRMT5 complex for methylation.1 WDR77 acts as a coactivator of androgen receptor transcription mediated through an androgen receptor-Smad1-WDR77 complex.2 High cytoplasmic and low nuclear levels of WDR77 are associated with the development of castration-resistant prostate cancer. Cayman’s WDR77 (human, recombinant) protein has a calculated molecular weight of 68 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Antonysamy, S. The structure and function of the PRMT5:MEP50 complex. Macromolecular protein complexes 185-194 (2017).

    2. Daniels, G., Jha, R., Shen, Y., et al. Androgen receptor coactivators that inhibit prostate cancer growth. Am. J. Clin. Exp. Urol. 5(1), 62-70 (2014).

    3. Abe, Y., Suzuki, Y., Kawamura, K., et al. MEP50/PRMT5-mediated methylation activates GLI1 in Hedgehog signalling through inhibition of ubiquitination by the ITCH/NUMB complex. Commun. Biol. 2, 23 (2019).