A fluorogenic substrate for plasmin
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D-Val-Leu-Lys-AMC (hydrochloride)

Item No. 44136

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
D-valyl-L-leucyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-L-lysinamide, hydrochloride
Synonyms
  • D-Val-L-Leu-L-Lys-7-amino-4-methylcoumarin
  • vLK-AMC
  • vLK-7-amino-4-methylcoumarin
Molecular Formula
C27H41N5O5 • XHCl
Formula Weight
515.7
Purity
≥98%
Emission
440-460 nm
Excitation
340-360 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: Sparingly soluble: 1-10 mg/mlEthanol: Slightly soluble: 0.1-1 mg/mlPBS (pH 7.2): Slightly soluble: 0.1-1 mg/ml
SMILES
CC(C)[C@@H](N)C(N[C@@H](CC(C)C)C(N[C@H](C(NC1=CC(OC(C=C2C)=O)=C2C=C1)=O)CCCCN)=O)=O.Cl
InChi Code
InChI=1S/C27H41N5O5.ClH/c1-15(2)12-21(32-27(36)24(29)16(3)4)26(35)31-20(8-6-7-11-28)25(34)30-18-9-10-19-17(5)13-23(33)37-22(19)14-18;/h9-10,13-16,20-21,24H,6-8,11-12,28-29H2,1-5H3,(H,30,34)(H,31,35)(H,32,36);1H/t20-,21-,24+;/m0./s1
InChi Key
PUDPBNYXPVKXPI-JZHGLUDASA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $28

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    5 mg

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    10 mg

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    25 mg

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    Product Description

    D-Val-Leu-Lys-AMC (vLK-AMC) is a fluorogenic substrate for plasmin.1 Upon enzymatic cleavage by plasmin, 7-amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify plasmin activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively. vLK-AMC has been used to quantify plasmin activity in primary human lung fibroblasts.2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Toul, M., Strunga, A., Damborsky, J., et al. Thrombolytic proteins profiling: High-throughput activity, selectivity, and resistance assays. FEBS Open Bio (2025).

    2. Zeitlmayr, S., Zierler, S., Staab-Weijnitz, C.A., et al. TRPM7 restrains plasmin activity and promotes transforming growth factor-β1 signaling in primary human lung fibroblasts. Arch. Toxicol. 96(10), 2767-2783 (2022).