Active • Host: E. coli • AA: 1-330 (full length) • Tag: N-terminal His • MW: 38.56 kDa
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Lipopolysaccharyl β-1,3-N-acetylgalactosaminyltransferase (H. influenzae F3031, recombinant)

Item No. 44171

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • LgtD
  • UDP-GlcNAc Lipooligosaccharide N-Acetylglucosamine Glycosyltransferase
Purity
≥90% estimated by SDS-PAGE
Source
Active recombinant H. influenzae N-terminal His-tagged LgtD expressed in E. coli
Amino Acids
1-323
MW
38.56 kDa
Formulation (Request formulation change)
50 mM Tris Hydrochloric acid, pH 7.5, with 50 mM sodium chloride, and 5 mM DTT
UniProt Accession №
E7A4Y7
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    5 mg

    $435

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    Cayman Chemical
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    Product Description

    Lipopolysaccharyl β-1,3-N-acetylgalactosaminyltransferase (LgtD) is an inverting N-acetylgalactosaminyltransferase and a member of glycosyltransferase family 2 (GT2) that is found in H. influenzae.1,2 A similar protein, lipopolysaccharide α-1,4-galactosyltransferase (LgtC) that is found in N. meningitidis, is composed of an N-terminal mixed α/β domain, which contains the active site, and a C-terminal helical domain, which is responsible for attachment to cell membranes.3,4 LgtD transfers an N-acetylgalactosamine from UDP-N-acetylgalactosamine (UDP-GalNAc) onto globotriose in the biosynthesis of the glycosphingolipid antigen globohexasaccharide (globoH) or globotetraose-containing LPS.5,6 Cayman's Lipopolysaccharyl β-1,3-N-acetylgalactosaminyltransferase (H. influenzae F3031, recombinant) protein can be used for enzyme activity assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Shao, J., Zhang, J., Kowal, P., et al. Overexpression and biochemical characterization of β-1,3-N-acetylgalactosaminyltransferase LgtD from Haemophilus influenzae strain Rd. Biochem. Biophys. Res. Commun. 295(1), 1-8 (2002).

    2. Campbell, J.A., Davies, G.J., Bulone, V., et al. A classification of nucleotide-diphospho-sugar glycosyltransferases based on amino acid sequence similarities. Biochem. J. 326(Pt 3), 929-939 (1997).

    3. Chang, K.H., Park, J.H., Chung, H.Y., et al. Enhanced expression of recombinant human cyclooxygenase 1 from stably-transfected Drosophila melanogaster S2 cells by dimethyl sulfoxide is mediated by up-regulation of nitric oxide synthase and transcription factor Kr-h1. Biotechnol. Lett. 34(7), 1243-1250 (2012).

    4. Persson, K., Ly, H.D., Dieckelmann, M., et al. Crystal structure of the retaining galactosyltransferase LgtC from Neisseria meningitidis in complex with donor and acceptor sugar analogs. Nat. Struct. Biol. 8(2), 166-175 (2001).

    5. Su, D.M., Eguchi, H., Yi, W., et al. Enzymatic synthesis of tumor-associated carbohydrate antigen Globo-H hexasaccharide. Org. Lett. 10(5), 1009-1012 (2008).

    6. Hood, D.W., Cox, A.D., Wakarchuk, W.W., et al. Genetic basis for expression of the major globotetraose-containing lipopolysaccharide from H. influenzae strain Rd (RM118). Glycobiology 11(11), 957-967 (2001).