Active • Host: HEK293 cells • AA: 22-839 • Tag: C-terminal His • MW: 95 kDa
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Leptin Receptor/CD295 Extracellular Domain (human, recombinant)

Item No. 44186

Product Insert (PDF)
Technical Information
Synonyms
  • Leptin Receptor
  • LEP-R
  • Obesity Receptor
  • OB-R
Purity
≥95% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/μg, determined by the LAL endotoxin assay
Source
Active recombinant human C-terminal His-tagged leptin receptor extracellular domain expressed in HEK293 cells
Amino Acids
22-839
MW
95 kDa
Formulation (Request formulation change)
Lyophilized from sterile PBS, pH 7.4
Host
HEK293
UniProt Accession №
P48357
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $298

    Add

    1 mg

    $1,937

    Add
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    Product Description

    Leptin receptor, also known as CD295, is a leptin-binding transmembrane receptor with roles in the regulation of food intake, energy homeostasis, and lipid metabolism.1,2 It is composed of a multi-domain extracellular region comprising an N-terminal domain, a cytokine receptor homology (CRH1) domain, an immunoglobulin-like (Ig-like) domain, a CRH2 domain that binds leptin, and a fibronectin type III (FNIII) domain, a transmembrane domain, and an intracellular signaling domain.1 The leptin receptor is encoded by LEPR in humans and undergoes alternative splicing to form six isoforms, all of which are expressed in a tissue-dependent manner, contain identical extracellular regions, and have variable intracellular domains.1,2,3 Leptin receptor deficiency is induced by mutations in LEPR and is associated with hypothyroidism, hypogonadotropic hypogonadism, growth hormone deficiency, and extreme early-onset obesity.4 Cayman’s Leptin Receptor/CD295 Extracellular Domain (human, recombinant) protein can be used for binding assay and Western blot (WB) applications. This protein consists of 829 amino acids, has a calculated molecular weight of 95 kDa, and a predicted N-terminus of Phe22 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is approximately 130-140 kDa due to glycosylation.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Berger, C., and Klöting, N. Leptin receptor compound heterozygosity in humans and animal models. Int. J. Mol. Sci. 22(9), 4475 (2021).

    2. Tartaglia, L.A. The leptin receptor. The Journal of Biological Chemisty 272(10), 6093-6096 (1997).

    3. Münzberg, H., Björnholm, M., Bates, S.H., et al. Leptin receptor action and mechanisms of leptin resistance. Cell Mol. Life Sci. 62(6), 642-652 (2005).

    4. Kleinendorst, L., Abawi, O., van der Kamp, H.J., et al. Leptin receptor deficiency: A systematic literature review and prevalence estimation based on population genetics. Eur. J. Endocrinol. 182(1), 47-56 (2020).