Host: E. coli • AA: 1-246 (full length) • MW: 28 kDa
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

14-3-3β (human, recombinant)

Item No. 44197

Product Insert (PDF)
Technical Information
Synonyms
  • 14-3-3 Protein Beta/Alpha
  • KCIP-1
  • PKC Inhibitor Protein 1
  • Protein Kinase C Inhibitor Protein 1
  • Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein β
  • YWHAB
Purity
≥90% estimated by SDS-PAGE
Source
Recombinant human 14-3-3β expressed in E. coli
Amino Acids
1-246 (full length)
MW
28 kDa
Formulation (Request formulation change)
Lyophilized from sterile 20 mM Tris, pH 7.5, with 150 mM sodium chloride, 0.1 mM DTT, and 10% glycerol
UniProt Accession №
P31946
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $298

    Add
    Cayman Chemical
    Visit Our Cancer Resource Center
    Find Tools & Resources to Study the Hallmarks of Cancer
    • Cancer cell signaling & regulation
    • Cancer metabolism
    • Tumor microenvironment
    EXPLORE NOW
    Product Description

    14-3-3β is a member of the 14-3-3 family of scaffolding proteins.1,2 It is composed of nine antiparallel α-helices, which contain one phosphopeptide binding site, and forms homodimers or heterodimers with other 14-3-3 isoforms to form a central channel with two ligand-binding grooves. 14-3-3β is ubiquitously expressed with the highest levels found in the brain.3,4 It is involved in diverse biological processes, including PKC activity regulation, axon regeneration, and cytoskeleton stabilization in podocytes.5,6,3 Increased intratumoral levels of 14-3-3β are associated with increasing tumor grade in patients with astrocytoma.7 Increased cerebrospinal fluid (CSF) levels of 14-3-3β are associated with Alzheimer’s disease.8 Cayman’s 14-3-3β (human, recombinant) protein consists of 248 amino acids, has a calculated molecular weight of 28 kDa, and two additional amino acids, Gly and Pro, at the N-terminus.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Obsilova, V., and Obsil, T. Structural insights into the functional roles of 14-3-3 proteins. Front. Mol. Biosci. 9, 1016071 (2022).

    2. Boston, P.F., Jackson, P., Kynoch, P.A.M., et al. Purification, properties, and immunohistochemical localisation of human brain 14-3-3 protein. J. Neurochem. 38, 1466-1474 (1982).

    3. Yasuda, H., Fukusumi, Y., Zhang, Y., et al. 14-3-3 Proteins stabilize actin and vimentin filaments to maintain processes in renal glomerular podocyte. The FASEB Journal 37(10), 23168 (2023).

    4. Boston, P.F., Jackson, P., and Thompson, R.J. Human 14-3-3 protein: radioimmunoassay, tissue distribution, and cerebrospinal fluid levels in patients with neurological disorders. J. Neurochem. 38(5), 1475-1482 (1982).

    5. Robinson, K., Jones, D., Patel, Y., et al. Mechanism of inhibition of protein kinase C by 14-3-3 isoforms. 14-3-3 isoforms do not have phospholipase A2 activity. Biochem. J. 299(3), 853-861 (1994).

    6. Kaplan, A., Morguette, B., Kroner, A., et al. Small-molecule stabilization of 14-3-3 protein-protein interactions stimulates axon regeneration. Neuron 93(5), 1082-1093 (2017).

    7. Yang, X., Cao, W., Lin, H., et al. Isoform-specific expression of 14-3-3 proteins in human astrocytoma. J. Neurol. Sci. 276(1-2), 54-59 (2009).

    8. Qiang, Q., Skudder-Hill, L., Toyota, T., et al. CSF 14-3-3β is associated with progressive cognitive decline in Alzheimer’s disease. Brain Commun. 5(6), fcad312 (2023).