BSA-NBD-Stearate Saturated Fatty Acid Complex (1 mM)

BSA-NBD-Stearate Saturated Fatty Acid Complex (1 mM) is composed of NBD-stearic acid (Item No. 10011299) and fatty acid-free bovine serum albumin (BSA) at an approximately 6:1 molar ratio of NBD-stearate:BSA. It displays excitation/emission maxima of 467/550 nm, respectively, and can be used to visualize lipid uptake using fluorescence microscopy or flow cytometry and to study the effect of long-chain fatty acids on lipid trafficking, fatty acid metabolism, and lipid droplet formation.^1,2,3 Cayman’s BSA-NBD-Stearate Saturated Fatty Acid Complex (1 mM) is suitable for use in short-term cell culture applications (acute treatment to 18 hours); however, for long-term applications (25+ hours) the product should be filter-sterilized using a 0.2 µm filter and sterile receptacle, which will not affect its performance. For best results, it is recommended that this product be used in conjunction with Cayman’s BSA Control for BSA-Fatty Acid Complexes (1 mM) (Item No. 34932), prepared with fatty acid-free BSA.

WARNING This product is not for human or veterinary use.

1. Weisiger, R.A. Saturable stimulation of fatty acid transport through model cytoplasm by soluble binding protein. Am. J. Physiol. 277(1), G109-G119 (1999).

2. Pohl, J., Ring, A., Ehehalt, R., et al. Long-chain fatty acid uptake into adipocytes depends on lipid raft function. Biochemistry 43(14), 4179-4187 (2004).

3. Murphy, E.J. Sterol carrier protein-2 expression increases NBD-stearate uptake and cytoplasmic diffusion in L cells. Am. J. Physiol. 275(2), G237-G243 (1998).