Active • Host: E. coli • AA: 2-663 (full length) • Tag: N-terminal His • MW: 75.74 kDa
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PAD4 (L117M mutant; human, recombinant)

Item No. 44266

Product Insert (PDF)
Technical Information
Synonyms
  • PADI4 rs1748033
  • PADI5 (L117M)
  • Protein Arginine Deiminase Type 4 (L117M)
  • Protein Arginine Deiminase Type IV (L117M)
Purity
≥80% estimated by SDS-PAGE
Source
Active recombinant human N-terminal His-tagged PAD4(L117M) expressed in E. coli
Amino Acids
2-663
MW
75.74 kDa
Formulation (Request formulation change)
50 mM Hepes, pH 8.0, with 300 mM sodium chloride, 1 mM DTT, and 10% glycerol
UniProt Accession №
Q9UM07
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    20 µg

    $160

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    100 µg

    $440

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    NET Formation Screening & Analysis Services
    Our experts are here to help.
    • Detection of NET formation ex vivo and screening for modulators using:
      • High-content imaging
      • Enzymatic detection
      • Citrullination/carbamylation detection
    • Experienced scientists skilled in neutrophil biology, isolation, and handling
    LEARN MORE
    Product Description

    Protein arginine deiminase 4 (PAD4) catalyzes the conversion of arginine residues to citrulline within cellular protein substrates, resulting in the loss of a positive charge, which can alter protein structure and/or function.1 It is expressed in neutrophils, as well as a variety of tissues, including the brain, liver, lung, and kidney.1,2,3 PAD4 has a key role in NETosis, a lytic form of cell death characterized by the release of neutrophil extracellular traps (NETs).1 Upon neutrophil activation, PAD4 translocates to the nucleus where it citrullinates histones, initiating chromatin decondensation and the release of NETs.2,4,5 A missense mutation in PADI4 leading to a leucine-to-methionine mutation at position 117 (PAD4L117M) is associated with increased susceptibility to rheumatoid arthritis.6,7,8 Cayman’s PAD4 (L117M mutant; human, recombinant) protein can be used for enzyme activity assay and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
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