A BSA complex mixture with cis-parinarate
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

BSA-cis-Parinarate Polyunsaturated Fatty Acid Complex (1 mM)

Item No. 44413

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Bovine Serum Albumin-cis-Parinarate
  • BSA-9(Z),11(E),13(E),15(Z)-Octadecatetraenoic Acid
Emission
432 nm
Excitation
320 nm
Formulation (Request formulation change)
1 mM cis-parinarate:0.17 mM BSA (6:1 cis-parinarate:BSA) in 150 mM sodium chloride, pH 7.4
Origin
Animal/Bovine
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    5 ml

    $182

    Add
    Lipid Resource Center
    Discover Products & Resources for Lipid Research
    • High-purity lipid standards
    • Lipid roles in biology
    • Lipids in health & disease
    • Lipids for pharmaceutical development
    • Protocols, advice, & resources
    EXPLORE NOW
    Product Description

    BSA-cis-Parinarate Polyunsaturated Fatty Acid Complex (1 mM) is composed of cis-parinaric acid (Item No. 71430) and fatty acid-free bovine serum albumin (BSA) at an approximately 6:1 molar ratio of cis-parinarate:BSA. It can be used for efficient fatty acid delivery to cells in culture for the purpose of monitoring lipid metabolism and inflammatory signaling pathways.1,2,3 In cell culture media, cis-parinaric acid displays excitation/emission maxima of 320/432 nm, respectively, and can be used to measure phospholipase and lipase activity or to monitor lipid peroxidation.4,5,6,7,8 Cayman’s BSA-cis-Parinarate Polyunsaturated Fatty Acid Complex (1 mM) is suitable for use in short-term cell culture applications (acute treatment to 18 hours); however, for long-term applications (25+ hours) the product should be filter-sterilized using a 0.2 µm filter and sterile receptacle, which will not affect its performance. For best results, it is recommended that this product be used in conjunction with Cayman's BSA Control for BSA-Fatty Acid Complexes (1 mM) (Item No. 34932), prepared with fatty acid-free BSA.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Spector, A.A., John, K., and Fletcher, J.E. Binding of long-chain fatty acids to bovine serum albumin. J. Lipid Res. 10(1), 56-67 (1969).

    2. Tuei, V.C., Ha, J.-S., and Ha, C.-E. Effects of human serum albumin complexed with free fatty acids on cell viability and insulin secretion in the hamster pancreatic β-cell line HIT-T15. Life Sci. 88(17-18), 810-818 (2011).

    3. Simard, J.R., Zunszain, P.A., Ha, C.-E., et al. Locating high-affinity fatty acid-binding sites on albumin by x-ray crystallography and NMR spectroscopy. Proc. Natl. Acad. Sci. U.S.A. 102(50), 17958-17963 (2005).

    4. Gomes, A., Fernandes, E., and Lima, J.L.F.C. Fluorescence probes used for detection of reactive oxygen species. J. Biochem. Biophys. Methods 65(2-3), 45-80 (2005).

    5. Wolf, C., Sagaert, L., and Bereziat, G. A sensitive assay of phospholipase using the fluorescent probe 2-parinaroyllecithin. Biochem. Biophys. Res. Commun. 99(1), 275-283 (1981).

    6. Beisson, F., Ferté, N., Nari, J., et al. Use of naturally fluorescent triacylglycerols from Parinari glaberrimum to detect low lipase activities from Arabidopsis thaliana seedlings. J. Lipid Res. 40(12), 2313-2321 (1999).

    7. McGuire, S.O., James-Kracke, M.R., Sun, G.Y., et al. An esterification protocol for cis-parinaric acid-determined lipid peroxidation in immune cells. Lipids 32(2), 219-226 (1997).

    8. de Hingh, Y.C.M., Meyer, J., Fischer, J.C., et al. Direct measurement of lipid peroxidation in submitochondrial particles. Biochemistry 34(39), 12755-12760 (1995).