Active • Host: E. coli • AA: 1-208 (full length) • Tag: N-terminal His • MW: 24.98 kDa
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Pyroglutamate Aminopeptidase (P. furiosus, recombinant)

Item No. 44452

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • 5-Oxoprolyl-peptidase
  • PGP-1
  • PGP-I
  • Pyrase
  • Pyroglutamyl-peptidase I
  • Pyrrolidone-carboxylate Peptidase
Purity
≥90% estimated by SDS-PAGE
Source
Active recombinant P. furiosus N-terminal His-tagged pyroglutamate aminopeptidase expressed in E. coli
Amino Acids
1-208
MW
25 kDa
Formulation (Request formulation change)
50 mM sodium phosphate buffer, pH 7.0, with 1 mM DTT and 1 mM EDTA
UniProt Accession №
O73944
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    0.1 U

    $599

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    Product Description

    Pyroglutamate aminopeptidase is a cysteine peptidase that cleaves pyroglutamic acid (pGlu) from the N-terminus of proteins and peptides.1 It is specific for L-pGlu-L-amino acid isomers and does not typically cleave pGlu-Pro bonds. Pyroglutamate aminopeptidase has been found in various species, including bacteria, mammals, fish, and birds and exists as a multimer in bacteria.1,2 Pyroglutamate aminopeptidase has commonly been used to remove pGlu from the N-terminus of peptides and proteins prior to Edman degradation and sequencing.3 Cayman’s Pyroglutamate Aminopeptidase (P. furiosus, recombinant) protein can be used for Edman degradation and protein sequencing applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Cummins, P.M., and O'Connor, B. Pyroglutamyl peptidase: An overview of the three known enzymatic forms. Biochim. Biophys. Acta 1429(1), 1-17 (1998).

    2. Dando, P.M., Fortunato, M., Strand, G.B., et al. Pyroglutamyl-peptidase I: Cloning, sequencing, and characterisation of the recombinant human enzyme. Protein Expr. Purif. 28(1), 111-119 (2003).

    3. Mozdzanowski, J., Bongers, J., and Anumula, K. High-yield deblocking of amino termini of recombinant immunoglobulins with pyroglutamate aminopeptidase. Anal. Biochem. 260(2), 183-187 (1998).