A polyhistidine peptide tag
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Product Type

Biochemicals
Peptides

6x-His (trifluoroacetate salt)

Item No. 44489

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
L-histidyl-L-histidyl-L-histidyl-L-histidyl-L-histidyl-L-histidine, trifluoroacetate salt
Synonyms
  • Hexa-His
  • His6
  • His-His-His-His-His-His-OH
Molecular Formula
C36H44N18O7 • XCF3COOH
Formula Weight
840.9
Purity
≥98%
Formulation (Request formulation change)
A solid
Peptide Sequence
HHHHHH-OH
Solubility(Learn about Variance in Solubility)
DMSO: Sparingly Soluble: 1-10 mg/mlEthanol: Sparingly Soluble: 1-10 mg/mlPBS pH 7.2: Soluble: ≥10 mg/ml
SMILES
N[C@@H](CC1=CN=CN1)C(N[C@@H](CC2=CN=CN2)C(N[C@@H](CC3=CN=CN3)C(N[C@H](C(N[C@H](C(N[C@H](C(O)=O)CC4=CN=CN4)=O)CC5=CN=CN5)=O)CC6=CN=CN6)=O)=O)=O.O=C(O)C(F)(F)F
InChi Code
InChI=1S/C36H44N18O7.C2HF3O2/c37-25(1-19-7-38-13-44-19)31(55)50-26(2-20-8-39-14-45-20)32(56)51-27(3-21-9-40-15-46-21)33(57)52-28(4-22-10-41-16-47-22)34(58)53-29(5-23-11-42-17-48-23)35(59)54-30(36(60)61)6-24-12-43-18-49-24;3-2(4,5)1(6)7/h7-18,25-30H,1-6,37H2,(H,38,44)(H,39,45)(H,40,46)(H,41,47)(H,42,48)(H,43,49)(H,50,55)(H,51,56)(H,52,57)(H,53,58)(H,54,59)(H,60,61);(H,6,7)/t25-,26-,27-,28-,29-,30-;/m0./s1
InChi Key
MAXXJEOKEOXUTK-FLQHCAAPSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    10 mg

    $35

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    25 mg

    $74

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    50 mg

    $123

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    100 mg

    $210

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    Product Description

    6x-His is a polyhistidine peptide tag.1 It has been used in various applications, including affinity purification, addition to the sequences of recombinant proteins to selectively pull-down those proteins, and structural biology applications.1,2,3,4

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Jung, K.-H., Park, Y.-S., Yeon, J.-H., et al. Improving the yield of soluble 6xHis-tagged interferon-α via the addition of repressor of the araBAD promoter system in Escherichia coli. Biotechnol. Lett. 30(9), 1577-1582 (2008).

    2. Crowe, J., Masone, B.S., and Ribbe, J. One-step purification of recombinant proteins with the 6xHis tag and Ni-NTA resin. Basic DNA and RNA protocols 58, 247-258 (1996).

    3. Karakus, C., Uslu, M., Yazici, D., et al. Evaluation of immobilized metal affinity chromatography kits for the purification of histidine-tagged recombinant CagA protein. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 1021, 182-187 (2016).

    4. Movellan, K.T., Zhu, W., Banks, D., et al. Expanding the tool box for native structural biology: 19F dynamic nuclear polarization with fast magic angle spinning. Sci. Adv. 10(40), eadq3115 (2024).