A synthetic bis-azo dye
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Evans Blue

Item No. 44513

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
6,6′-[(3,3′-dimethyl[1,1′-biphenyl]-4,4′-diyl)bis(2,1-diazenediyl)]bis[4-amino-5-hydroxy-1,3-naphthalenedisulfonic acid, tetrasodium salt
CAS Number
314-13-6
Synonyms
  • Direct Blue 53
  • T-1824
Molecular Formula
C34H24N6O14S4 • 4Na
Formula Weight
960.8
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: Soluble: ≥10 mg/mlPBS pH 7.2: Soluble: ≥10 mg/ml
SMILES
[O-]S(=O)(C(C=C1S([O-])(=O)=O)=C2C(C(O)=C(C=C2)N=NC(C(C)=C3)=CC=C3C4=CC=C(N=NC5=C(C6=C(N)C(S([O-])(=O)=O)=CC(S([O-])(=O)=O)=C6C=C5)O)C(C)=C4)=C1N)=O.[Na+].[Na+].[Na+].[Na+]
InChi Code
InChI=1S/C34H28N6O14S4.4Na/c1-15-11-17(3-7-21(15)37-39-23-9-5-19-25(55(43,44)45)13-27(57(49,50)51)31(35)29(19)33(23)41)18-4-8-22(16(2)12-18)38-40-24-10-6-20-26(56(46,47)48)14-28(58(52,53)54)32(36)30(20)34(24)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
InChi Key
ATNOAWAQFYGAOY-UHFFFAOYSA-J
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    100 mg

    $25

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    500 mg

    $119

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    Product Description

    Evans blue is a synthetic bis-azo dye.1 It displays an absorbance maximum at 620 nm. Evans blue has been used for the estimation of blood volume and determination of blood-brain barrier integrity, among other applications.1,2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Yao, L., Xue, X., Yu, P., et al. Evans blue dye: A revisit of its applications in biomedicine. Contrast Media & Mol. Imaging 2018, 7628037 (2018).

    2. Goldim, M.P.S., Della Giustina, A., and Petronilho, F. Using evans blue dye to determine blood-brain barrier integrity in rodents. Curr. Protec. Immunol. 126(1), e83 (2019).