A xanthene dye
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Eosin B

Item No. 44529

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
6′-dihydroxy-2′,7′-dinitro-4′,5′-dibromo-3′ spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, disodium salt
CAS Number
548-24-3
Synonyms
  • Acid Red 91
  • C.I. 45400
  • Dibromodinitrofluorescein
  • Eosin Bluish
Molecular Formula
C20H6Br2N2O9 • 2Na
Formula Weight
624.1
Emission
544 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
DMSO: Soluble: ≥10 mg/mlEthanol: Sparingly soluble: 1-10 mg/mlPBS: Soluble: ≥10 mg/ml
SMILES
O=C1OC2(C3=CC([N+]([O-])=O)=C(C(Br)=C3OC4=C(C([O-])=C([N+]([O-])=O)C=C42)Br)[O-])C5=C1C=CC=C5.[Na+].[Na+]
InChi Code
InChI=1S/C20H8Br2N2O9.2Na/c21-13-15(25)11(23(28)29)5-9-17(13)32-18-10(6-12(24(30)31)16(26)14(18)22)20(9)8-4-2-1-3-7(8)19(27)33-20;;/h1-6,25-26H;;/q;2*+1/p-2
InChi Key
QGAYMQGSQUXCQO-UHFFFAOYSA-L
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

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    Product Description

    Eosin B is a xanthene dye and brominated derivative of fluorescein.1,2,3 It displays absorption/emission maxima of 514/544 nm, respectively.2,3 Eosin B has been used as an alternative to eosin Y (Item No. 40128) in hematoxylin and eosin (H&E) staining to stain the extracellular matrix and cytoplasmic proteins.4 It inhibits the growth of P. falciparum (IC50 = 124 nM).1

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Massimine, K.M., McIntosh, M.T., Doan, L.T., et al. Eosin B as a novel antimalarial agent for drug-resistant Plasmodium falciparum. Antimicrob. Agents Chemother. 50(9), 3132-3141 (2006).

    2. Kato, H., Komagoe, K., Nakanishi, Y., et al. Xanthene dyes induce membrane permeabilization of bacteria and erythrocytes by photoinactivation. Photochem. Photobiol. 88(2), 423-431 (2012).

    3. Sabnis, R.W. Handbook of biological dyes and stains: Synthesis and industrial applications. (2010).

    4. Feldman, A.T., and Wolfe, D. Tissue processing and hematoxylin and eosin staining. Methods Mol. Biol. 1180, 31-43 (2014).