For depleting dead and dying cells from biological samples while maintaining the health and physiology of viable cells of inerest
Features
  • Contains 1 ml of dead cell removal microbubbles
  • Fast and easy workflow effectively removes dead cells in 25 minutes
  • Isolated viable cells are ready for flow sorting, single-cell sequencing, pharmacologic studies, or other downstream assays
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Dead Cell Removal Microbubble Kit

Item No. 44554

Technical Information
Synonyms
  • BACS™ Microbubbles
Shipping & Storage Information
Storage
4°C
Shipping
Wet ice in continental US; may vary elsewhere
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    Product Description

    Spend less time preparing your sample and more time analyzing it with our 25-minute, industry-leading microbubble protocol. BACS™ Dead Cell Removal Microbubbles eliminate dead cells, enabling better science with more isolated, viable cells in a cleaner sample.

    Mechanism of Action: During apoptosis, phosphatidylserine (PS) – a phospholipid normally found on the inner surface of the plasma membrane – is redistributed to the extracellular surface. The relocation of PS to the outer surface of the cell membrane provides an effective target for differentiating dead cells from viable cells within a biological sample. The Dead Cell Removal Kit utilizes BACS™ Microbubbles coated with Annexin V, a PS binding molecule, to capture and float dead cells away.

    How it works: The Dead Cell Removal Microbubble Kit are mixed into the sample where they bind to dead and dying cells. Using their inherent buoyancy, the microbubbles quickly and gently float the dead cells to the surface. Following a centrifugation step, the microbubbles and dead cells are aspirated away leaving the viable cells of interest untouched and ready for downstream applications.

    Cell Type: Multiple

    Sample Species and Source: Multiple

    Cell Separation Method: Negative

    Capacity: 1 ml of Annexin V BACS™ Microbubbles

    Sample Size: 500k-50M cells

    Kit Includes:

    • Dead cell removal microbubbles

    • Dead cell removal binding buffer

    • 1M CaCl<2

    WARNING This product is not for human or veterinary use.