Active • Host: Insect cells • AA: 1-1,368 (full length) • Tag: C-terminal His • MW: 163 kDa
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Product Type

Proteins
Enzymes

Cas9 (serotype M1) (S. pyogenes, recombinant; His-tagged, 3xNLS)

Item No. 44669

Product Insert (PDF)
Technical Information
Synonyms
  • CRISPR-associated endonuclease Cas9/Csn1
  • SpCas9
  • SpyCas9
Purity
≥90% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/μg, determined by the LAL endotoxin assay
Source
Active recombinant S. pyogenes N-terminal NLS-containing and C-terminal 2xNLS-containing and His-tagged Cas9 expressed in insect cells
Amino Acids
1-1,368
MW
163 kDa
Formulation (Request formulation change)
20 mM Tris, pH 8.0, 300 mM sodium, 20% glycerol, 0.5 mM PMSF, 0.1 mM EDTA, and 0.5 mM TCEP
Host
Insect cells
UniProt Accession №
Q99ZW2
Shipping & Storage Information
Storage
-80°C
Shipping
Wet ice in continental US; may vary elsewhere
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Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    200 µg

    $388

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    Product Description

    CRISPR-associated protein 9 (Cas9) is an RNA-guided DNA endonuclease that is part of the bacterial and archaeal CRISPR/CRISPR-Cas system, which provides immune defense against viruses and plasmids.1 It is composed of nuclease (NUC) and recognition (REC) lobes connected by an arginine-rich bridge helix (BH).2 The NUC lobe contains one HNH and three RuvC endonuclease domains and a protospacer-adjacent motif-interacting domain (PAM-interacting domain), while the REC lobe contains three recognition domains involved in Cas9 RNA- and DNA binding. Cas9 is involved in the type II CRISPR/Cas system that uses CRISPR RNA (crRNA) and trans-activating crRNA (tracrRNA) to guide Cas9 to foreign DNA, where it introduces double-stranded breaks in a site-specific manner.1 The CRISPR/Cas9 system, with and without modifications to Cas9, has been harnessed to edit genomes in vitro and in vivo.3,4 Cayman's Cas9 (serotype M1) (S. pyogenes, recombinant; His-tagged, 3xNLS) protein contains an N-terminal nuclear localization signal (NLS), two C-terminal NLSs, and a C-terminal His tag, consists of 1,407 amino acids, and has a calculated molecular weight of 163 kDa. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is approximately 140 kDa, which may be due to faster migration from compact folding or glycosylation effects.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Jinek, M., Chylinski, K., Fonfara, I., et al. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337(6096), 816-821 (2012).

    2. Babu, K., Kathiresan, V., Kumari, P., et al. Coordinated actions of Cas9 HNH and RuvC nuclease domains are regulated by the bridge helix and the target DNA sequence. Biochemistry 60(49), 3783-3800 (2021).

    3. Li, T., Yang, Y., Qi, H., et al. CRISPR/Cas9 therapeutics: Progress and prospects. Signal Transduct. Target Ther. 8(1), 36 (2023).

    4. Karimi, M.A., Paryan, M., Behrouzian Fard, G., et al. Challenges and opportunities in the application of CRISPR-Cas9: A review on genomic editing and therapeutic potentials. Med. Princ. Pract. 17, 1-17 (2025).