A BSA-fatty acid complex and paired control for use in cell culture
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BSA-Palmitate Reagent Set (5 mM)

Item No. 44698

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Bovine Serum Albumin-PA
  • Bovine Serum Albumin-Palmitate
  • BSA-Hexadecanoic Acid
  • BSA-PA
Formulation (Request formulation change)
Item No. 44699: 5 mM Palmitate:0.8 mM BSA (6:1 palmitate:BSA) in 150 mM sodium chloride, pH 7.4; Item No. 44700: 0.8 mM BSA in 150 mM sodium chloride, pH 7.4
Origin
Animal/Bovine
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    1 ea

    $296

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    Product Description

    The BSA-Palmitate Reagent Set (5 mM) contains a BSA-palmitate complex solution and a fatty acid-free BSA control solution. BSA-Palmitate is composed of palmitic acid (Item No. 10006627) and fatty acid-free bovine serum albumin (BSA) at an approximately 6:1 molar ratio of palmitate:BSA. The BSA-palmitate complex and BSA control were prepared at the same time with the same lot of fatty acid-free BSA under sterile conditions, then filtered and aliquoted into sterile vials. The BSA-Palmitate Reagent Set (5 mM) can be used for efficient fatty acid delivery to cells in culture for the purpose of monitoring lipid metabolism, including fatty acid oxidation, and inflammatory signaling pathways, such as in studies of obesity-related inflammation and insulin resistance.1,2,3 It can also be used to study the effect of long-chain fatty acid uptake on gene and protein expression, lipid droplet formation, hepatocyte lipid accumulation, and oxidative stress.4,5,6 Cayman's BSA-Palmitate Reagent Set (5 mM) is suitable for use in short- and long-term cell culture applications (25+ hours).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Alsabeeh, N., Chausse, B., Kakimoto, P.A., et al. Cell culture models of fatty acid overload: Problems and solutions. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1863(2), 143-151 (2018).

    2. Wang, D., Green, M.F., McDonnell, E., et al. Oxygen flux analysis to understand the biological function of sirtuins. Methods Mol. Biol. 1077, 241-258 (2013).

    3. Radin, M.S., Sinha, S., Bhatt, B.A., et al. Inhibition or deletion of the lipopolysaccharide receptor Toll-like receptor-4 confers partial protection against lipid-induced insulin resistance in rodent skeletal muscle. Diabetologia 51(2), 336-346 (2008).

    4. Muthusamy, G., Liu, C.-C., and Johnston, A.N. Deletion of PGAM5 downregulates FABP1 and attenuates long-chain fatty acid uptake in hepatocellular carcinoma. Cancers (Basel) 15(19), 4796 (2023).

    5. Das, S., Finney, A.C., Anand, S.K., et al. Inhibition of hepatic oxalate overproduction ameliorates metabolic dysfunction-associated steatohepatitis. Nat. Metab. 6(10), 1939-1962 (2024).

    6. Guilfoyle-Speese, A., Patel, K., Ghanwat, A.H., et al. Short-chain fatty acids and palmitate induce distinct metabolic and phenotypic signatures in normal and ischemic skeletal muscle microvascular endothelial cells. Cells 15(6), 493 (2026).