For immunochemical detection of MARCH6
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MARCH6 Monoclonal Antibody (Clone 26F12C)

Item No. 44928

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Doa10 Homolog
  • E3 Ubiquitin-protein Ligase MARCH6
  • MARCHF6
  • MARCH-VI
  • Membrane-associated RING-CH Protein VI
  • Membrane-associated RING-CH-type Finger 6
  • Membrane-associated RING Finger (C3HC4) 6
  • Membrane-associated RING Finger Protein 6
  • RING Finger Protein 176
  • RNF176
  • TEB4
Immunogen
Recombinant human MARCH6C9A
Clone Designation
26F12C
Formulation (Request formulation change)
100 µg of protein G-purified monoclonal antibody
Storage Buffer
PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide
Host
Mouse
Isotype
IgG1K
Applications
IF, WB
Cross Reactivity
(+) MARCH6
Species Reactivity
(+) Human
UniProt Accession №
O60337
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $425

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    Product Description

    Membrane-associated RING-CH-type finger 6 (MARCH6) is an E3 ubiquitin ligase.1,2 It is composed of an N-terminal C4HC3-type RING domain, which has catalytic activity, and 14 transmembrane domains. MARCH6 is ubiquitously expressed and localizes to the endoplasmic reticulum (ER).1,3 It plays a role in ER-associated degradation (ERAD), where it targets substrates involved in cholesterol synthesis and trafficking, lipid droplet formation, bile salt transport, thyroid hormone activation, and G-protein signaling for proteasomal degradation.1,4 MARCH6 is also involved NADPH sensing, ferroptosis, and redox homeostasis.5 Intratumoral expression of MARCHF6 is increased in a variety of cancers, and intronic TTTTA/TTTCA repeat expansions in MARCHF6 are associated with familial adult myoclonic epilepsy (FAME).5,6 Cayman’s MARCH6 Monoclonal Antibody (Clone 26F12C) can be used for immunofluorescence (IF) and Western blot (WB) applications. The antibody recognizes MARCH6 at approximately 75 kDa from human samples using 4-20% SDS-PAGE.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lin, H., Li, S., and Shu, H.-B. The membrane-associated MARCH E3 ligase family: Emerging roles in immune regulation. Front. Immunol. 10, 1751 (2019).

    2. Samji, T., Hong, S., and Means, R.E. The membrane associated RING-CH proteins: A family of E3 ligases with diverse roles through the cell. Int. Sch. Res. Notices 2014, 637295 (2014).

    3. Xu, S., Donnelly, L., Kober, D.L., et al. Development of a monoclonal antibody to study MARCH6, an E3 ligase that regulates proteins that control lipid homeostasis. J. Lipid Res. 65(11), 100650 (2024).

    4. Scott, N.A., Sharpe, L.J., and Brown, A.J. The E3 ubiquitin ligase MARCHF6 as a metabolic integrator in cholesterol synthesis and beyond. Biochim. Biophys. Acta Mol. Cell Biol. Lipids 1866(1), 158837 (2021).

    5. Zhen, Y., Chen, S., Niu, B., et al. Unraveling MARCH6’s role in cancer progression and metabolism from protein homeostasis to oncogenesis. Pharmacol. Res. 229, 108236 (2026).

    6. Florian, R.T., Kraft, F., Leitão, E., et al. Unstable TTTTA/TTTCA expansions in MARCH6 are associated with familial adult myoclonic epilepsy type 3. Nat. Commun. 10(1), 4919 (2019).