Active • Host: Insect cells • AA: 2-1,169 (full length) • MW: 128.4 kDa
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Rabankyrin-5 (human, recombinant)

Item No. 45169

Product Insert (PDF)
Technical Information
Synonyms
  • Ankyrin Repeat and FYVE Domain-containing Protein 1
  • Ankyrin Repeats Hooked To A Zinc Finger Motif
  • Rank-5
Purity
≥90% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/μg, determined by the LAL endotoxin assay
Source
Active recombinant human Rabankyrin-5 expressed in insect cells
Amino Acids
2-1,169 (full length)
MW
128.4 kDa
Formulation (Request formulation change)
50 mM Tris-HCl, pH 7.5, with 200 mM sodium chloride and 20% glycerol
Host
Insect cells
UniProt Accession №
Q9P2R3
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    10 µg

    $282

    Add

    50 µg

    $776

    Add
    Product Description

    Rabankyrin-5 is a Rab5 effector protein with roles in endosomal fusion, micropinocytosis, and mitochondrial homeostasis and dynamics.1,2 It is composed of an N-terminal BTB domain, 21 ankyrin (ANK) repeats, and a C-terminal FYVE finger domain and primarily localizes to endosomal membranes, but can also localize to the cytosol.1 Rabankyrin-5 localizes with Rab5-positive early endosomes, and immunodepletion of Rabankyrin-5 using anti-Rabankyrin-5 antibodies inhibits early endosome fusion in HeLa cell cytosol. Knockdown of ANKFY1, the gene encoding Rabankyrin-5, using endoribonuclease-prepared small interfering RNA (esiRNA) inhibits EGF-induced micropinocytosis in skin cancer cells. ANKFY1 knockdown also induces mitochondrial elongation and stasis in retinal pigment epithelial (RPE) cells.3 Cayman’s Rabankyrin-5 (human, recombinant) protein has a calculated molecular weight of 128.4 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Schnatwinkel, C., Christoforidis, S., Lindsay, M.R., et al. The Rab5 effector Rabankyrin-5 regulates and coordinates different endocytic mechanisms. PLoS Biol. 2(9), E261 (2004).

    2. Farmer, T., Naslavsky, N., and Caplan, S. Tying trafficking to fusion and fission at the mighty mitochondria. Traffic 19(8), 569-577 (2018).

    3. Farmer, T., Reinecke, J.B., Xie, S., et al. Control of mitochondrial homeostasis by endocytic regulatory proteins. J. Cell. Sci. 130(14), 2359-2370 (2017).