Active • Host: E. coli • AA: 2-592 (full length) • MW: 67.3 kDa
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BRAP (human, recombinant)

Item No. 45174

Product Insert (PDF)
Technical Information
Synonyms
  • BRAP23
  • BRCA1-associated protein
  • Impedes Mitogenic Signal Propagation
  • IMP
  • Renal Carcinoma Antigen NY-REN-63
  • RING Finger Protein 52
  • RING-type E3 Ubiquitin Transferase BRAP2
Purity
≥90% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/g, determined by the LAL endotoxin assay
Source
Active recombinant human BRAP expressed in E. coli
MW
67.3 kDa
Formulation (Request formulation change)
50 mM Tris-HCl, pH 7.5, containing 200 mM sodium chloride and 20% glycerol
UniProt Accession №
Q7Z569
Shipping & Storage Information
Shipping
in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    10 µg

    $235

    Add

    50 µg

    $588

    Add
    Product Description

    BRCA1-associated protein (BRAP) is an E3 ubiquitin ligase with roles in the regulation of nuclear protein import and cell signaling.1 It is composed of a nucleotide-binding α/β plait, RING-finger, UBP-like zinc-finger (ZfUBP), and coiled-coil domain and localizes to the cytoplasm.1,2 BRAP binds to a variety of transcription factors and kinases in the cytosol to both inhibit nuclear translocation and impede signal propagation. SNPs in BRAP are associated with an increased risk of myocardial infarction and with a predisposition to alcohol use disorder.3,4 Cayman’s BRAP (human, recombinant) protein can be used for enzyme activity and Western blot (WB) applications and has a calculated molecular weight of 67.3 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Shoji, S., Hanada, K., Ohsawa, N., et al. Central catalytic domain of BRAP (RNF52) recognizes the types of ubiquitin chains and utilizes oligo-ubiquitin for ubiquitylation. Biochem. J. 474(18), 3207-3226 (2017).

    2. Fulcher, A.J., Roth, D.M., Fatima, S., et al. The BRCA-1 binding protein BRAP2 is a novel, negative regulator of nuclear import of viral proteins, dependent on phosphorylation flanking the nuclear localization signal. The FASEB Journal 24(5), 1454-1466 (2010).

    3. Ozaki, K., Sato, H., Inoue, K., et al. SNPs in BRAP associated with risk of myocardial infarction in Asian populations. Nat. Genet. 41(3), 329-333 (2009).

    4. Kim, J.W., Choe, Y.M., Shin, J.G., et al. Associations of BRAP polymorphisms with the risk of alcohol dependence and scores on the alcohol use disorders identification test. Neuropsychiatr. Dis. Treat. 15, 83-94 (2018).