Active • Host: Insect cells • AA: 2-776 (cullin 1) (full length); 1-108 (RBX1) (full length) • Tag: N-terminal His-Strep II • MW: 92.8 (cullin 1) and 12.3 (RBX1) kDa
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Cullin 1/RBX1 Complex (human, recombinant)

Item No. 45182

Product Insert (PDF)
Technical Information
Synonyms
  • Cullin 1
  • CUL1
  • Cul-1
  • RBX1
  • E3 Ubiquitin-protein Ligase RBX1
  • E3 Ubiquitin-protein Transferase RBX1
  • Regulator of Cullins-1
  • RING Box Protein 1
  • RING Finger Protein 75
  • RNF75
  • ROC1
  • Protein ZYP
Purity
≥90% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/g, determined by the LAL endotoxin assay
Source
Active recombinant human N-terminal His-Strep II-tagged cullin 1/RBX1 complex expressed in insect cells
Amino Acids
2-776 (cullin 1) (full length)/1-108 (RBX1)
MW
92.8 kDa (cullin 1)/12.3 (RBX1) kDa
Formulation (Request formulation change)
50 mM Tris-HCl, pH 7.5, with 200 mM sodium chloride, 20% glycerol, and 1 mM DTT
UniProt Accession №
Q13616/P62877
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    10 µg

    $249

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    50 µg

    $623

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    Cayman Chemical
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    Product Description

    The cullin 1 RING ligase (CRL1) complex is an E3 ubiquitin ligase complex.1 It is composed of an F-box protein linked to S-phase kinase-associated protein 1 (SKP1) that determines substrate specificity, cullin 1, and RING box protein 1 (RBX1) or RBX2, which binds to the ubiquitin-loaded E2.2 Cullin 1 acts as a scaffold that interacts with SKP1 via its N-terminal stalk domain and RBX1 or RBX2 at its C-terminal globular domain.3 NEDDylation of cullin 1 alters its conformation and increases the affinity of the complex for certain E2 enzymes.4 The CRL1 complex is involved in a variety of biological processes, including embryogenesis, cell cycle progression, and the DNA damage response.2,1,5 CUL1, the gene encoding cullin 1, and RBX1 are overexpressed in a variety of cancers and associated with poor overall survival.6,7,8,9 Cayman’s Cullin 1/RBX1 Complex (human, recombinant) can be used for enzyme activity assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Zhou, W., Wei, W., and Sun, Y. Genetically engineered mouse models for functional studies of SKP1-CUL1-F-box-protein (SCF) E3 ubiquitin ligases. Cell Res. 23(5), 599-619 (2013).

    2. Xie, J., Jin, Y., and Wang, G. The role of SCF ubiquitin-ligase complex at the beginning of life. Reprod. Biol. Endocrinol. 17(1), 101 (2019).

    3. Zheng, N., Schulman, B.A., Song, L., et al. Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex. Nature 416(6882), 703-709 (2004).

    4. Saha, A., and Deshaies, R.J. Multimodal activation of the ubiquitin ligase SCF by Nedd8 conjugation. Mol. Cell 32(1), 21-31 (2008).

    5. Silverman, J.S., Skaar, J.R., and Pagano, M. SCF ubiquitin ligases in the maintenance of genome stability. Trends Biochem. Sci. 37(2), 66-73 (2012).

    6. Wang, W., Qiu, J., Liu, Z., et al. Overexpression of RING box protein-1 (RBX1) associated with poor prognosis of non-muscle-invasive bladder transitional cell carcinoma. J. Surg. Oncol. 107(7), 758-761 (2013).

    7. Xing, R., Chen, K.-B., Xuan, Y., et al. RBX1 expression is an unfavorable prognostic factor in patients with non-small cell lung cancer. Surg. Oncol. 25(3), 147-151 (2016).

    8. Jiang, H., He, D., Xu, H., et al. Cullin-1 promotes cell proliferation via cell cycle regulation and is a novel in prostate cancer. Int. J. Clin. Exp. Pathol. 8(2), 1575-1583 (2015).

    9. Zhou, Y.H., Xia, J., Xu, W.H., et al. Cullin-1 promotes cell proliferation in human breast cancer and is related to diabetes. Int. J. Biol. Markers 31(4), e375-e381 (2016).