Active • Host: Insect cells • AA: 2-745 (cullin 2) (full length), 1-108 (RBX1) (full length) • Tag: N-terminal His-Strep II • MW: 90.1 (cullin 2) and 12.3 (RBX1) kDa
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Cullin 2/RBX1 Complex (human, recombinant)

Item No. 45183

Product Insert (PDF)
Technical Information
Synonyms
  • CUL2
  • E3 Ubiquitin-protein Ligase RBX1
  • E3 Ubiquitin-protein Transferase RBX1
  • Protein ZYP
  • Regulator of Cullins-1
  • RING Box Protein 1
  • RING Finger Protein 75
  • RNF75
  • ROC1
Purity
≥90% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/μg, determined by the LAL endotoxin assay
Amino Acids
2-745/1-108
MW
90.1 (cullin 2) kDa/12.3 (RBX1) kDa
Formulation (Request formulation change)
50 mM HEPES, pH 7.5, with 200 mM sodium chloride, 20% glycerol, and 1 mM DTT
UniProt Accession №
Q13617/P62877
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    100 µg

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    Cayman Chemical
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    Product Description

    The cullin 2 RING ligase (CRL2) complex is an E3 ubiquitin ligase complex.1,2 It is composed of a von Hippel-Lindau (VHL) E3 ligase, a substrate recognition receptor that interacts with elongin B and -C, cullin 2 as the scaffold, and RING box protein 1 (RBX1), which binds to ubiquitin-loaded E2.1 NEDDylation of cullin 2 alters the conformation of cullin 2 and RBX1 to promote ubiquitin transfer to the CRL2 substrate.2 The CRL2 complex is involved in germline development and viral infection.1 A SNP in CUL2, the gene encoding cullin 2, is associated with an increased risk for Crohn’s disease and ulcerative colitis.3 Cayman’s Cullin 2/RBX1 Complex (human, recombinant) can be used for enzyme activity assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Cai, W., and Yang, H. The structure and regulation of Cullin 2 based E3 ubiquitin ligases and their biological functions. Cell Div. 11, 7 (2016).

    2. Boh, B.K., Smith, P.G., and Hagen, T. Neddylation-induced conformational control regulates cullin RING ligase activity in vivo. J. Mol. Biol. 409(2), 136-145 (2011).

    3. Rivas, M.A., Beaudoin, M., Gardet, A., et al. Deep resequencing of GWAS loci identifies independent rare variants associated with inflammatory bowel disease. Nat. Genet. 43(11), 1066-1073 (2011).