Intracellular Cytokine Staining Preparation Reagent Kit

The components of this kit, when combined with an appropriate cell culture medium, enable detection of cytokine production upon cellular activation.^1,2 Phorbol 12-myristate 13-acetate (PMA), a PKC activator, together with the calcium ionophore ionomycin, provide a strong activating stimulus to memory T cells and other immune cells in short-term (approximately 4 hours) culture. With addition of the Golgi transport inhibitor brefeldin A during the last hour of stimulation, the cytokines produced in response to PMA and ionomycin accumulate in the cells.²

Each component in this kit is provided separately, and instructions are included to reconstitute these reagents for dilution into cell culture media. This kit provides the flexibility to optimize stimulation conditions to your specifications. Prior to use, reagents must be stored as indicated at -20°C.

WARNING This product is not for human or veterinary use.

1. Chatila, T., Silverman, L., Miller, R., et al. Mechanisms of T cell activation by the calcium ionophore ionomycin. J. Immunol. 143(4), 1283-1289 (1989).

2. Prussin, C. Cytokine flow cytometry: Understanding cytokine biology at the single-cell level. J. Clin. Immunol. 17(3), 195-204 (1997).