HyNic NHS ester

HyNic NHS ester is a heterobifunctional linker molecule.¹ It contains an amine-reactive N-hydroxysuccinimide (NHS) moiety and a hydrazine moiety that reacts with the aldehyde functional group in SFB (succinimidyl 4-formylbenzoate; Item No. 30477) to form a bis-aryl hydrazone linker that is quantifiable spectrophotometrically at 354 nm. HyNic NHS ester has been used in the synthesis of an antibody-drug conjugate (ADC) that contains the toll-like receptor 7 (TLR7) agonist UC-1V150 and the anti-CD20 monoclonal antibody rituximab and increases pro-inflammatory activity in RAW 264.7 macrophages.² HyNic NHS ester has also been used in the formation of polymer-enzyme, protein-liposome, and peptide-peptide conjugates.^1,3,4

WARNING This product is not for human or veterinary use.

1. Grotzky, A., Manaka, Y., Kojima, T., et al. Preparation of catalytically active, covalent α-polylysine-enzyme conjugates via UV/vis-quantifiable bis-aryl hydrazone bond formation. Biomacromolecules 12(1), 134-144 (2011).

2. Gadd, J.R., Greco, F., Cobb, A.J.A., et al. Targeted activation of toll-Like receptors: Conjugation of a toll-like receptor 7 agonist to a monoclonal antibody maintains antigen binding and specificity. Bioconjug. Chem. 26(8), 1743-1752 (2015).

3. Gardner, M.W., and Brodbelt, J.S. Ultraviolet photodissociation mass spectrometry of bis-aryl hydrazone conjugated peptides. Anal. Chem. 81(12), 4864-4872 (2009).

4. Nagata, H., Yoshimoto, M., and Walde, P. Preparation and catalytic properties of carbonic anhydrase conjugated to liposomes through a bis-aryl hydrazone bond. ACS Omega 8(21), 18637-17652 (2023).